Nutrient resupplementation arrests bio-oil accumulation in Phaeodactylum tricornutum.
Nutrient resupplementation arrests bio-oil accumulation in Phaeodactylum tricornutum.
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DOI：
10.1007/s00253-013-5010-y
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发表时间：
2013-08
影响因子：
5
通讯作者：
Fields, M. W.
中科院分区：
文献类型：
--
作者：
Valenzuela, J.;Carlson, R. P.;Gerlach, R.;Cooksey, K.;Peyton, B. M.;Bothner, B.;Fields, M. W.
三角褐指藻是硅藻纲的一种海洋硅藻，对海洋初级生产力和生物燃料生产中的脂肪积累分别具有重要的生态和工业意义。三酰甘油酯(TAG)在不同营养胁迫下的积累已被报道，我们的结果表明，在硝酸盐或磷酸盐耗尽的情况下，可能会发生脂肪积累。然而，根据尼罗红测定法和脂肪酸甲酯(FAME)谱的观察，当这两种营养物质都被耗尽时，观察到更大的脂肪积累。硝酸盐耗竭对脂肪积累的影响大于磷酸盐耗竭。利用耗尽的营养物质进行再补充时，三角帆船体内的脂肪积累被阻止。在硝酸盐再补充后，耗尽氮的细胞显示出从脂质积累模式到细胞生长模式的明显转变，通过增加细胞数量和消耗积累的脂肪来观察到这一转变。磷酸盐耗竭导致脂肪积累，这种积累在磷酸盐再补充时被阻止。停止脂肪堆积之后是脂肪的消耗，但细胞数量没有增加。同时耗尽硝酸盐和磷酸盐的细胞在添加硝酸盐和磷酸盐时显示出细胞生长，并且在重新补充时具有最大的观察到的脂肪消耗。这些结果表明，与硝酸盐再补充不同，磷酸盐再补充可以阻止脂肪积累，但不会导致细胞转移到细胞生长。这些数据表明，营养再补充将阻止脂肪积累，细胞生长和脂肪积累之间的切换可以根据氮和磷的可用性进行调节。本文的在线版本(doi：10.1007/s00253-0135010-y)包含补充材料，授权用户可以使用。
Phaeodactylum tricornutum is a marine diatom in the class Bacillariophyceae and is important ecologically and industrially with regards to ocean primary production and lipid accumulation for biofuel production, respectively. Triacylglyceride (TAG) accumulation has been reported in P. tricornutum under different nutrient stresses, and our results show that lipid accumulation can occur with nitrate or phosphate depletion. However, greater lipid accumulation was observed when both nutrients were depleted as observed using a Nile Red assay and fatty acid methyl ester (FAME) profiles. Nitrate depletion had a greater effect on lipid accumulation than phosphate depletion. Lipid accumulation in P. tricornutum was arrested upon resupplementation with the depleted nutrient. Cells depleted of nitrogen showed a distinct shift from a lipid accumulation mode to cellular growth post-resupplementation with nitrate, as observed through increased cell numbers and consumption of accumulated lipid. Phosphate depletion caused lipid accumulation that was arrested upon phosphate resupplementation. The cessation of lipid accumulation was followed by lipid consumption without an increase in cell numbers. Cells depleted in both nitrate and phosphate displayed cell growth upon the addition of both nitrate and phosphate and had the largest observed lipid consumption upon resupplementation. These results indicate that phosphate resupplementation can shut down lipid accumulation but does not cause cells to shift into cellular growth, unlike nitrate resupplementation. These data suggest that nutrient resupplementation will arrest lipid accumulation and that switching between cellular growth and lipid accumulation can be regulated upon the availability of nitrogen and phosphorus. The online version of this article (doi:10.1007/s00253-013-5010-y) contains supplementary material, which is available to authorized users.
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DOI：
10.1139/b05-077
发表时间：
2005-07-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
Granum, E;Raven, JA;Leegood, RC
通讯作者：
Leegood, RC
影响因子：
5
作者：
Mus, Florence;Toussaint, Jean-Paul;Carlson, Ross P.
通讯作者：
Carlson, Ross P.
影响因子：
3.8
作者：
Aslan, Sebnem;Kapdan, Ilgi Karapinar
通讯作者：
Kapdan, Ilgi Karapinar
不同硝酸盐和磷酸盐浓度下佐芬小球藻的脂质积累和生长特性。
影响因子：
2.8
作者：
Feng, Pingzhong;Deng, Zhongyang;Hu, Zhengyu
通讯作者：
Hu, Zhengyu
影响因子：
7
作者：
Lee, Do Yup;Park, Jeong-Jin;Fiehn, Oliver
通讯作者：
Fiehn, Oliver