The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe
The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe
复制标题
Plo1激酶在粟酒裂殖酵母有丝分裂定型和分隔中的作用
作者：
Kayoko Tanaka;Janni Petersen;Fiona H. MacIver;Daniel P. Mulvihill;D. Glover;I. Hagan
Plo1相关的酪蛋白激酶活性在分裂前的有丝分裂期间达到顶峰。磷酸酶处理取消了这一活性。有丝分裂Plo1的激活需要预先激活M期促进因子(MPF)，这表明在有丝分裂承诺过程中，Plo1并不作为有丝分裂触发蛋白来启动MPF的激活。由于Plo1Δ细胞不能分离，以及在Plo1+过表达后大量的分离，提示了Plo1与隔膜起始网络(SIN)之间的联系。Spg1是调节SIN活性的G蛋白，它的间期激活诱导了隔膜，但不刺激Plo1相关蛋白的活性。相反，SIN失活并不影响Plo1相关激酶活性的有丝分裂刺激。在36℃时，plo1.ts4细胞形成一个畸形的肌动蛋白环，但很少分离。强制激活Spg1使突变型plo1.ts4细胞，但不能使Sin组分Sid2缺陷的细胞将肌动蛋白环转化为隔膜。过表达pl1+诱导隔膜的能力被SIN失活严重削弱。我们认为，Plo1在罪之前起作用，以控制隔膜。
Plo1‐associated casein kinase activity peaked during mitosis before septation. Phosphatase treatment abolished this activity. Mitotic Plo1 activation had a requirement for prior activation of M‐phase promoting factor (MPF), suggesting that Plo1 does not act as a mitotic trigger kinase to initiate MPF activation during mitotic commitment. A link between Plo1 and the septum initiating network (SIN) has been suggested by the inability of plo1Δ cells to septate and the prolific septation following plo1+ overexpression. Interphase activation of Spg1, the G protein that modulates SIN activity, induced septation but did not stimulate Plo1‐associated kinase activity. Conversely, SIN inactivation did not affect the mitotic stimulation of Plo1‐associated kinase activity. plo1.ts4 cells formed a misshapen actin ring, but rarely septated at 36°C. Forced activation of Spg1 enabled plo1.ts4 mutant cells, but not cells with defects in the SIN component Sid2, to convert the actin ring to a septum. The ability of plo1+ overexpression to induce septation was severely compromised by SIN inactivation. We propose that Plo1 acts before the SIN to control septation.
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影响因子：
10.5
作者：
Bridge, AJ;Morphew, M;Hagan, IM
通讯作者：
Hagan, IM
影响因子：
4
作者：
J. Mitchison;P. Nurse
通讯作者：
J. Mitchison;P. Nurse
影响因子：
3.3
作者：
IZUMI, T;MALLER, JL
通讯作者：
MALLER, JL
新裂殖酵母胞质分裂突变体的分离和表征。
影响因子：
3.3
作者：
Balasubramanian,MK;McCollum,D;Chang,L;Wong,KC;Naqvi,NI;He,X;Sazer,S;Gould,KL
通讯作者：
Gould,KL
影响因子：
56.9
作者：
Qian, YW;Erikson, E;Maller, JL
通讯作者：
Maller, JL