The genetic, morphological, and physiological characterization of a dark larval cuticle mutation in the butterfly, Bicyclus anynana.
The genetic, morphological, and physiological characterization of a dark larval cuticle mutation in the butterfly, Bicyclus anynana.
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DOI：
10.1371/journal.pone.0011563
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发表时间：
2010-07-14
期刊：
影响因子：
3.7
通讯作者：
Monteiro A
中科院分区：
文献类型：
--
作者：
Bear A;Simons A;Westerman E;Monteiro A
对昆虫黑化病的研究有助于我们理解自然选择和影响黑色素表型进化的最终因素。对几种黑鳞翅类幼虫的研究发现，低水平的循环幼体激素（JH）滴度与黑色素表型相关，这表明JH生物合成途径的遗传变化导致该群体角质层中黑色素颗粒沉积增加。但是，在不同的物种中，黑变是通过不同的分子机制产生的吗？本研究报道了一种双环蝇（鳞翅目：蛱蝶科）深色幼虫单位点突变，其幼虫表皮颜色较野生型深。不像其他鳞翅类的黑化幼虫突变，这是常染色体隐性遗传，似乎不涉及JH滴度的缺陷。与JH缺乏突变体不同，深色幼虫突变体的生长速度和性行为与野生型相似，局部应用JH类似物未能挽救野生型的表皮颜色。最后，透射电子显微镜显示，在该物种的角质层中，黑色素的硬化或弥漫性黑色素的沉积，而不是黑色素颗粒的沉积，产生了深色。我们得出结论，不同种类鳞翅目幼虫黑化的分子机制不同。
Studies on insect melanism have greatly contributed to our understanding of natural selection and the ultimate factors influencing the evolution of darkly pigmented phenotypes. Research on several species of melanic lepidopteran larvae have found that low levels of circulating juvenile hormone (JH) titers are associated with a melanic phenotype, suggesting that genetic changes in the JH biosynthetic pathway give rise to increased deposition of melanin granules in the cuticle in this group. But does melanism arise through different molecular mechanisms in different species? The present study reports on a Bicyclus anynana (Lepidoptera: Nymphalidae) dark larvae single locus mutation, in which larvae exhibit a darker cuticle relative to wild type. Unlike other lepidopteran melanic larvae mutations, this one is autosomal recessive and does not appear to involve a deficiency in JH titers. Unlike JH deficiency mutants, dark larvae mutants display similar growth rates and sexual behaviors as wild type, and topical application of a JH analogue failed to rescue the wild type cuticular coloration. Finally, transmission electron microscopy showed that sclerotization or deposition of diffuse melanin, rather than deposition of melanin granules, produces the dark coloration found in the cuticle of this species. We conclude that different molecular mechanisms underlie larval melanism in different species of Lepidoptera.
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DOI：
10.1073/pnas.0336368100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Wittkopp, PJ;Williams, BL;Carroll, SB
通讯作者：
Carroll, SB
影响因子：
2.2
作者：
SAFRANEK, L;RIDDIFORD, LM
通讯作者：
RIDDIFORD, LM
影响因子：
4.9
作者：
Pool, John E.;Aquadro, Charles F.
通讯作者：
Aquadro, Charles F.
影响因子：
2.2
作者：
TRUMAN, JW;RIDDIFORD, LM;SAFRANEK, L
通讯作者：
SAFRANEK, L
影响因子：
64.8
作者：
Joron, M;Brakefield, PM
通讯作者：
Brakefield, PM