Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs.
Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs.
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DOI：
10.4142/jvs.2007.8.3.275
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发表时间：
2007-09
影响因子：
1.8
通讯作者：
Kweon OK
中科院分区：
文献类型：
--
作者：
Lim JH;Byeon YE;Ryu HH;Jeong YH;Lee YW;Kim WH;Kang KS;Kweon OK
本研究旨在探讨同种异体脐带血（UCB）来源的间充质干细胞（MSCs）和重组甲硫氨酰人粒细胞集落刺激因子（rmhGCSF）对犬第一腰椎球囊压迫脊髓损伤模型的影响。根据脊髓损伤后的治疗，将25只成年杂种犬分为5组：未治疗（CN）;盐水治疗（CP）; rmhGCSF治疗（G）; UCB-MSC治疗（UCB-MSC）;联合治疗（UCBG）。将从犬胎儿的脐带血分离的UCB-MSC制备为106个细胞/150 μl盐水。将UCB-MSCs直接注射到脊髓损伤部位，并在诱导脊髓损伤后1周皮下注射rmhGCSF。采用Olby评分、磁共振成像、体感诱发电位和组织病理学检查评价移植后功能恢复情况。在0周评价时，所有组的Olby评分均为0。移植后2周，UCB-MSC组和UCBG组的Olby评分显著高于CN组和CP组。然而，UCB-MSC和UCBG组之间以及CN和CP组之间没有显著差异。这些比较在移植后4周和8周保持稳定。体感诱发电位神经传导速度明显改善。此外，在UCB-MSC和UCBG组的脊髓损伤中注意到神经细胞体的明显结构一致性。这些结果表明，移植UCB-MSCs导致脊髓损伤犬的神经功能恢复，并可被视为脊髓损伤的治疗方式。
This study was to determine the effects of allogenic umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) and recombinant methionyl human granulocyte colony-stimulating factor (rmhGCSF) on a canine spinal cord injury model after balloon compression at the first lumbar vertebra. Twenty-five adult mongrel dogs were assigned to five groups according to treatment after a spinal cord injury: no treatment (CN); saline treatment (CP); rmhGCSF treatment (G); UCB-MSCs treatment (UCB-MSC); co-treatment (UCBG). The UCB-MSCs isolated from cord blood of canine fetuses were prepared as 106 cells/150 µl saline. The UCB-MSCs were directly injected into the injured site of the spinal cord and rmhGCSF was administered subcutaneously 1 week after the induction of spinal cord injury. The Olby score, magnetic resonance imaging, somatosensory evoked potentials and histopathological examinations were used to evaluate the functional recovery after transplantation. The Olby scores of all groups were zero at the 0-week evaluation. At 2 week after the transplantation, the Olby scores in the groups with the UCB-MSC and UCBG were significantly higher than in the CN and CP groups. However, there were no significant differences between the UCB-MSC and UCBG groups, and between the CN and CP groups. These comparisons remained stable at 4 and 8 week after transplantation. There was significant improvement in the nerve conduction velocity based on the somatosensory evoked potentials. In addition, a distinct structural consistency of the nerve cell bodies was noted in the lesion of the spinal cord of the UCB-MSC and UCBG groups. These results suggest that transplantation of the UCB-MSCs resulted in recovery of nerve function in dogs with a spinal cord injury and may be considered as a therapeutic modality for spinal cord injury.
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影响因子：
2.4
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通讯作者：
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