Comparison of T Helper Cell Patterns in Primary Open-Angle Glaucoma and Normal-Pressure Glaucoma.
Comparison of T Helper Cell Patterns in Primary Open-Angle Glaucoma and Normal-Pressure Glaucoma.
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原发性开角型青光眼和常压性青光眼中 T 辅助细胞模式的比较
DOI：
10.12659/msm.904923
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发表时间：
2018-04-04
期刊：
影响因子：
--
通讯作者：
Guo W
中科院分区：
文献类型：
--
作者：
Guo C;Wu N;Niu X;Wu Y;Chen D;Guo W
研究背景正常眼压性青光眼（normal-pressure glaucoma，NPG）患者存在HSP 60相关的免疫活性，而原发性开角型青光眼（primaryopen-angleglaucoma，POAG）患者的眼压没有升高。HSP 60在POAG和NPG患者中均有表达，而抗HSP 60抗体主要在NPG患者中表达增高。本研究的目的是比较Th细胞的百分比和相关细胞因子的水平，试图提供证据来解释这种差异。材料/方法收集POAG、NPG和正常对照组（NC）的外周血，分离单核细胞，在有或无HSP 60刺激下培养。采用流式细胞术和酶联免疫吸附试验检测培养前后Th 1、Th 2、Th 17和Treg细胞的百分比，以及HSP 60抗体水平和相关细胞因子水平。结果NPG患者血清中抗HSP 60抗体滴度明显高于正常对照组。POAG和NPG患者的Th 1、Th 2细胞频率、IL-4水平和IFN-γ水平相当，而Treg细胞频率仅在POAG患者中较高。HSP 60培养后，POAG、NPG和NC组Th 2细胞频率升高，Th 1细胞频率降低，Treg细胞频率仅在POAG和NC组升高。结论原发性开角型青光眼、非原发性开角型青光眼和正常对照组Th细胞类型不同。部分NPG患者存在Treg细胞缺乏诱导，Th细胞促炎和抗炎反应模式失衡。
Background HSP60-related immunological activities are found in normal-pressure glaucoma (NPG) patients, in whom an elevated intraocular pressure (IOP) found in primary open-angle glaucoma (POAG) is not observed. HSP60 was found in POAG and NPG patients, while anti-HSP60 level was mainly found to be higher in NPG patients. The purpose of this study was to compare the percentages of Th cells and levels of related cytokines, attempting to provide evidence to explain this discrepancy. Material/Methods Blood samples from POAG, NPG, and normal control (NC) groups were collected and peripheral blood monocytes were isolated and cultured with or without the stimulation of HSP60. Flow cytometry and enzyme-linked immunosorbent assay were used to assess the percentages of Th1, Th2, Th17, and Treg cells, as well as HSP60 antibody levels and related cytokine levels, before and after culture. Results Significantly higher titers of anti-HSP60 were observed only in NPG patients. Comparable Th1 and Th2 cell frequencies, IL-4 level, and IFN-γ level were found in POAG and NPG patients, while higher Treg cell frequency was only found in POAG patients. After culturing with HSP60, increased Th2 frequencies and decreased Th1 frequencies were observed in the POAG, NPG, and NC groups, while increased Treg frequency was only identified in the POAG and NC groups. Conclusions Different Th cell patterns were observed among POAG, NPG, and NC groups. Lack of induction of Treg cells and imbalance of the pro-inflammatory and anti-inflammatory response patterns of Th cells exist in some NPG patients.
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DOI：
10.1073/pnas.0408197102
发表时间：
2005-01-11
影响因子：
11.1
作者：
Chen, ML;Pittet, MJ;Khazaie, K
通讯作者：
Khazaie, K
影响因子：
13.7
作者：
Iwase, Aiko;Araie, Makoto;Kitazawa, Yoshiaki
通讯作者：
Kitazawa, Yoshiaki
影响因子：
2
作者：
Joachim, S. C.;Wax, M. B.;Grus, F. H.
通讯作者：
Grus, F. H.
影响因子：
3.5
作者：
Liu, Yuanyuan;You, Caiyun;Yan, Hua
通讯作者：
Yan, Hua
影响因子：
32.4
作者：
Mempel, Thorsten R.;Pittet, Mikael J.;von Andrian, Ulrich H.
通讯作者：
von Andrian, Ulrich H.