RepD-mediated recruitment of PcrA helicase at the Staphylococcus aureus pC221 plasmid replication origin, oriD.
RepD-mediated recruitment of PcrA helicase at the Staphylococcus aureus pC221 plasmid replication origin, oriD.
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DOI：
10.1093/nar/gkp1153
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发表时间：
2010-04
影响因子：
14.9
通讯作者：
Soultanas P
中科院分区：
文献类型：
--
作者：
Machón C;Lynch GP;Thomson NH;Scott DJ;Thomas CD;Soultanas P
质粒编码复制起始（Rep）蛋白将宿主解旋酶招募到质粒复制起点。在此之前，我们发现RepD定向地将pcr解旋酶招募到pC221 oriD上，保持与它的关联，并在质粒解绕过程中增加其加工能力。研究表明，RepD在核心oriD的上游和下游形成了一个复合体。RepD的结合导致了核心区域上游的一个区域的重塑，形成了PcrA的“着陆点”。这个扩展的RepD - dna复合体通过与上游位点的RepD的相互作用招募PcrA。即使在没有RepD的情况下，PcrA对该区域的亲和力也很弱。在ADPNP （ATP的非水解类似物）被PcrA结合后，RepD-PcrA-ATP起始复合物的构象重排将其严格限制在核心oriD的边界内。我们得出结论，repd介导的oriD上的PcrA募集是一个三步过程。首先，扩展的RepD-oriD复合体包括核心oriD上游的区域；第二，PcrA被招募到上游区域，第三，结合atp的PcrA在核心区域内重新定位。
Plasmid encoded replication initiation (Rep) proteins recruit host helicases to plasmid replication origins. Previously, we showed that RepD recruits directionally the PcrA helicase to the pC221 oriD, remains associated with it, and increases its processivity during plasmid unwinding. Here we show that RepD forms a complex extending upstream and downstream of the core oriD. Binding of RepD causes remodelling of a region upstream from the core oriD forming a ‘landing pad’ for the PcrA. PcrA is recruited by this extended RepD–DNA complex via an interaction with RepD at this upstream site. PcrA appears to have weak affinity for this region even in the absence of RepD. Upon binding of ADPNP (non-hydrolysable analogue of ATP), by PcrA, a conformational rearrangement of the RepD–PcrA–ATP initiation complex confines it strictly within the boundaries of the core oriD. We conclude that RepD-mediated recruitment of PcrA at oriD is a three step process. First, an extended RepD–oriD complex includes a region upstream from the core oriD; second, the PcrA is recruited to this upstream region and thirdly upon ATP-binding PcrA relocates within the core oriD.
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影响因子：
5.6
作者：
HIGASHITANI, A;GREENSTEIN, D;HORIUCHI, K
通讯作者：
HORIUCHI, K
影响因子：
5.6
作者：
THOMAS, CD;NIKIFOROV, TT;SHAW, WV
通讯作者：
SHAW, WV
影响因子：
3.4
作者：
Schuck, P
通讯作者：
Schuck, P
影响因子：
5.6
作者：
IORDANESCU, S;BASHEER, R
通讯作者：
BASHEER, R
DOI：
10.1007/bf00280216
发表时间：
1993-10-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
IORDANESCU, S
通讯作者：
IORDANESCU, S