Mosquito transgenic technologies to reduce Plasmodium transmission.

Mosquito transgenic technologies to reduce Plasmodium transmission.
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减少疟原虫传播的蚊子转基因技术。

DOI:
10.1007/978-1-62703-026-7_41
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发表时间:
2013
影响因子:
--
通讯作者:
A. Crisanti
A. Crisanti
中科院分区:
--
文献类型:
--
作者:
S. Fuchs;T. Nolan;A. Crisanti

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将选择的遗传构建体引入按蚊基因组的能力为研究疟原虫寄生虫与其昆虫宿主之间的分子相互作用提供了有价值的工具。从长远来看,这项技术有可能通过抑制目标蚊子种群或通过防止病原体传播的性状渐渗来提供控制病媒传播疾病的新方法。在这里,我们详细描述了用于生成转基因冈比亚按蚊蚊子的生殖系转化的基础上,使用修改的转座因子或位点特异性PhiC31重组酶的协议。
The ability to introduce genetic constructs of choice into the genome of Anopheles mosquitoes provides a valuable tool to study the molecular interactions between the Plasmodium parasite and its insect host. In the long term, this technology could potentially offer new ways to control vector-borne diseases through the suppression of target mosquito populations or through the introgression of traits that preclude pathogen transmission. Here, we describe in detail protocols for the generation of transgenic Anopheles gambiae mosquitoes based on germ-line transformation using either modified transposable elements or the site-specific PhiC31 recombinase.
DOI: 10.1016/s0965-1748(00)00110-7
发表时间: 2000-11-01
影响因子: 3.8
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期刊: GENETICS
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影响因子: 3.3
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