SOS-independent mutagenesis in lacZ induced by methylene blue plus visible light
SOS-independent mutagenesis in lacZ induced by methylene blue plus visible light
复制标题
亚甲蓝加可见光诱导的 lacZ 中不依赖于 SOS 的诱变
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
S. Boiteux
中科院分区：
文献类型：
--
作者：
B. Tudek;J. Laval;S. Boiteux
在亚甲蓝（MB-光）存在下通过可见光的体外光敏化在M13 mpl 8lacZ噬菌体DNA中产生损伤，在转染到各种细菌宿主中后分析其致死和致突变潜力。使用M13 mp 18的lacZ基因作为靶标，通过正向突变试验确定突变。当MB光处理的双链（ds）M13 mp 18 DNA用于转染未诱导SOS功能的野生型细胞时，与未处理的DNA相比，在10%存活率下观察到突变频率增加了5倍。MB-光处理的ds M13 mp 18 DNA的突变频率是更大的转染到uvrA的fpg-1双突变体比在uvrA，fpg-1，或umuC单突变体或野生型。序列分析表明，在野生型菌株中，MB-光处理的ds M13 mp 18 DNA的结果主要是单碱基替换。最常见的碱基变化是GC→TA颠换。MB-光处理单链（ss）M13 mp 18 DNA也导致转染到野生型菌株后突变频率增加，主要产生G→T颠换。我们的研究结果表明，MB-光诱导的诱变是至少部分独立的SOS功能在大肠杆菌中的诱导。突变谱表明，8-氧代-7，8-二氢鸟嘌呤是DNA的主要致突变损伤。
SummaryIn vitro photosensitization by visible light in the presence of methylene blue (MB-light) produces lesions in M13mpl8 lacZ phage DNA, the lethal and mutagenic potential of which was analyzed after transfection into various bacterial hosts. Mutagenesis was determined with a forward mutation assay using the lacZ gene of M13mp18 as a target. When, MB-light-treated double-stranded (ds) M13mp18 DNA was used to transfect wild-type cells which were not induced for SOS functions, a fivefold increase in mutation frequency was observed at 10% survival compared to that observed with untreated DNA. Mutation frequency obtained with MB-light-treated ds M13mp18 DNA was greater when transfected into the uvrA fpg-1 double mutant than that seen in uvrA, fpg-1, or umuC single mutants or in the wild-type. Sequence analysis shows that in the wild-type strain, MB-light treatment of ds M13mp18 DNA results mostly in single base substitutions. The most frequent base change is the GC→TA transversion. MB-light treatment of single-stranded (ss) M13mp18 DNA also results in an increased mutation frequency after transfection into the wild-type strain, yielding mostly G→T transversions. Our results show that MB-light-induced mutagenesis is at least partially independent of the induction of SOS functions in Escherichia coli. The mutation spectra suggest that 8-oxo-7,8-dihydroguanine is the major promutagenic lesion in DNA.
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影响因子：
5.6
作者：
ELLEDGE, SJ;WALKER, GC
通讯作者：
WALKER, GC
DOI：
10.1016/s0021-9258(19)39637-1
发表时间：
1990-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Kaspar;W. Rychlik;M. White;R. Rhoads;D. Morris
通讯作者：
R. Kaspar;W. Rychlik;M. White;R. Rhoads;D. Morris
影响因子：
16.6
作者：
G. C. Walker
通讯作者：
G. C. Walker
影响因子：
2.9
作者：
WOOD, ML;DIZDAROGLU, M;ESSIGMANN, JM
通讯作者：
ESSIGMANN, JM
DOI：
10.1073/pnas.88.11.4690
发表时间：
1991-06-01
影响因子：
11.1
作者：
TCHOU, J;KASAI, H;NISHIMURA, S
通讯作者：
NISHIMURA, S