The neurotoxic effect of isoflurane on age-defined neurons generated from tertiary dentate matrix in mice.
The neurotoxic effect of isoflurane on age-defined neurons generated from tertiary dentate matrix in mice.
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异氟醚对小鼠第三齿状基质产生的年龄限定神经元的神经毒性作用
DOI：
10.1002/brb3.1949
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发表时间：
2021-01
影响因子：
3.1
通讯作者：
Liu JX
中科院分区：
文献类型：
--
作者：
Xiao XL;Wu JT;Zhang HZ;Wang YD;Zhang JQ;Liu LF;Yu-Chen;Min-Li;Yang PB;Wu XL;Liu JX
近年来的动物实验表明，异氟醚暴露可导致海马神经发生障碍和认知功能障碍。然而，关于异氟烷暴露对第三齿状回基质产生的神经元的影响知之甚少，尽管婴儿时期颗粒细胞数量的大量增加主要来自该区域。为了标记来自第三齿状突基质的新细胞，在出生后第6天（P6）给小鼠注射BrdU。然后，在注射BrdU后1、8、21和42天，将小鼠暴露于异氟烷4小时，并在24小时后收集脑。分别通过BrdU、BrdU + DCX、BrdU + NeuN或BrdU + Prox-1染色评估具有不同发育阶段的新生成细胞/神经元的损失。我们发现，异氟烷暴露显着减少新生细胞（1天）和成熟神经元（42天）的数量，但对未成熟（8天）和早期成熟神经元（8和21天）没有影响。结果表明，异氟烷暴露对小鼠中具有年龄定义模式的第三齿状突基质起源细胞产生神经毒性作用，这部分解释了异氟烷暴露于年轻大脑导致的认知障碍。异氟烷暴露对小鼠中具有年龄定义模式的三级齿状突基质来源细胞产生神经毒性作用。异氟烷显著减少了从三级齿状回基质驱动的新生细胞和成熟神经元的数量。
Recent animal studies showed that isoflurane exposure may lead to the disturbance of hippocampal neurogenesis and later cognitive impairment. However, much less is known about the effect of isoflurane exposure on the neurons generated form tertiary dentate matrix, even though a great increase of granule cell population during the infantile period is principally derived from this area. To label the new cells originated from the tertiary dentate matrix, the mice were injected with BrdU on postnatal day 6 (P6). Then, the mice were exposed to isoflurane for 4 hr at 1, 8, 21, and 42 days after BrdU injection, and the brains were collected 24 hr later. The loss of newly generated cells/neurons with different developmental stage was assessed by BrdU, BrdU + DCX, BrdU + NeuN, or BrdU + Prox‐1 staining, respectively. We found that the isoflurane exposure significantly decreased the numbers of nascent cells (1 day old) and mature neurons (42 days old), but had no effect on the immature (8 days old) and early mature neurons (8 and 21 days old, respectively). The results suggested isoflurane exposure exerts the neurotoxic effects on the tertiary dentate matrix‐originated cells with an age‐defined pattern in mice, which partly explain the cognitive impairment resulting from isoflurane exposure to the young brain. Isoflurane exposure exerts neurotoxic effects on the tertiary dentate matrix‐originated cells with an age‐defined pattern in mice. The isoflurane significantly decreased the numbers of nascent cells and mature neurons driven from the tertiary dentate matrix.
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影响因子：
3.7
作者：
Lin N;Moon TS;Stratmann G;Sall JW
通讯作者：
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影响因子：
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作者：
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作者：
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影响因子：
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作者：
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