Diversity analysis of streptomycetes and associated phosphotranspherase genes in soil.
Diversity analysis of streptomycetes and associated phosphotranspherase genes in soil.
复制标题
DOI：
10.1371/journal.pone.0035756
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wellington EM
中科院分区：
文献类型：
--
作者：
Laskaris P;Sekine T;Wellington EM
根据分离工作，试图验证灰色链霉菌在土壤中普遍存在的观察结果。基于管家基因atpD建立了链霉菌属特异性PCR，并用于研究土壤中链霉菌的物种多样性。S.研究了在同一土壤中链霉素生产菌与仅具有抗性的链霉素磷酸转移酶（strA）的共存情况。开发了另外两种基于PCR的测定;一种特异于与生产相关的strA，另一种特异于更多样化的strA和其他相关的磷酸转移酶。两个S。atpD和strA基因在所有检测土壤中均低于PCR检测限。许多更多样化的磷酸转移酶基因被扩增，其中少数可能与链霉素的生产。我们的结论是，无论是链霉素生产商还是S。灰霉菌在新鲜或甲壳素和淀粉改良的土壤中普遍存在（少于土壤放线菌的0.1%）。其中一个土壤地点接受了植物霉素（活性成分：链霉素），多样性研究表明，这改变了土壤中的链霉素种群。
An attempt was made to verify the observation that Streptomyces griseus was prevalent in soil based on isolation work. A genus-specific PCR was developed for Streptomyces based on the housekeeping gene atpD and used to investigate species diversity within selected soils. The presence of S. griseus was investigated to determine coexistence of resistance-only streptomycin phosphotransferase (strA) in the same soil as streptomycin producers. Two additional PCR-based assays were developed; one specific for strA in association with production, the other for more diverse strA and other related phosphotranferases. Both the S. griseus atpD and strA genes were below the PCR detection limit in all soils examined. A number of more diverse phosphotransferase genes were amplified, a minority of which may be associated with streptomycin production. We conclude that neither streptomycin producers nor S. griseus are prevalent in the fresh or chitin and starch-amended soils examined (less than 0.1% of soil actinobacteria). One of the soil sites had received plantomycin (active ingredient: streptomycin) and diversity studies suggested that this altered the streptomycete populations present in the soil.
登录
查看更多内容
DOI：
10.1023/a:1010296220929
发表时间：
2001-06-01
影响因子：
2.6
作者：
Egan, S;Wiener, P;Wellington, EMH
通讯作者：
Wellington, EMH
影响因子：
5.1
作者：
Laskaris, Paris;Tolba, Sahar;Wellington, Liz
通讯作者：
Wellington, Liz
DOI：
10.1007/s10482-007-9146-6
发表时间：
2007-08-01
影响因子：
2.6
作者：
Goodfellow, Michael;Kumar, Yashawant;Sembiring, Langkah
通讯作者：
Sembiring, Langkah
DOI：
10.1007/s10295-005-0077-9
发表时间：
2006-07-01
影响因子：
3.4
作者：
Baltz, Richard H.
通讯作者：
Baltz, Richard H.
影响因子：
5.1
作者：
Chanal, A;Chapon, V;Heulin, T
通讯作者：
Heulin, T