The effect of a mutagen (N-methyl-N-nitro-N-nitrosoguanidine) on cultured cells from adult Schistosoma japonicum

The effect of a mutagen (N-methyl-N-nitro-N-nitrosoguanidine) on cultured cells from adult Schistosoma japonicum
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诱变剂(N-甲基-N-硝基-N-亚硝基胍)对日本血吸虫成虫培养细胞的影响

DOI:
10.1007/s00436-005-0083-x
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发表时间:
2006-04
影响因子:
2
通讯作者:
Zhong, QP
Zhong, QP
中科院分区:
医学3区
文献类型:
--
作者:
Dong, HF;Grevelding, CG;Ming, ZP;Jiang, MS;Zhong, QP

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采用单因素试验和正交试验研究了N-甲基-N-硝基-N-亚硝基胍(MNNG)对日本血吸虫培养细胞的转化作用。在MNNG的影响下,培养的细胞生长良好,在低血清培养基中细胞存活时间超过246天。用3 μg/ml MNNG处理48 h,明视野和扫描电镜(SEM)观察到细胞分裂数明显增加。在此条件下,诱导细胞表面出现丰富的微绒毛、皱褶、微脊、乳头和大泡。
The transforming effect of N-methyl-N-nitro-N-nitrosoguanidine (MNNG) on cultured cells from Schistosoma japonicum (S.japonicum) was studied using mono-factor and orthogonal tests. Under the influence of MNNG, cultured cells grew well, and cell survival time was more than 246 days in low-serum medium. When treated with 3 μg/ml MNNG for 48 h, the number of dividing cells increased significantly as determined by bright-field and scanning electron microscopy (SEM). Under these conditions, abundant microvilli, ruffles, microridges, papillae and blebs were observed on the surface of the induced cells.
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