No re-calibration required? Stability of a bioelectrochemical sensor for biodegradable organic matter over 800 days.
No re-calibration required? Stability of a bioelectrochemical sensor for biodegradable organic matter over 800 days.
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DOI：
10.1016/j.bios.2021.113392
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发表时间：
2021-10-15
影响因子：
12.6
通讯作者：
Head IM
中科院分区：
文献类型：
--
作者：
Spurr MW;Yu EH;Scott K;Head IM
微生物燃料电池（MFC）作为生物传感器运行可能使真正低成本，实时监测废水中的有机负荷。由MFC产生的电流已经与有机负荷的常规测量（例如生化需氧量（BOD））相关联，但是在这种传感器的可靠性和适用性方面仍有很多有待建立。在这项研究中，分批模式和多级，流动模式的MFC运行超过800天，并定期重新校准与合成废水含有葡萄糖和谷氨酸（GGA）。通过将测量的电流归一化为最大电流的百分比来获得BOD 5校准曲线。在重新校准之间几乎没有漂移，并且组合数据集的非线性Hill模型的R2为88- 95%，随着时间的推移和跨设备表现出稳定的响应。尽管如此，也评估了影响校准的因素。增加外电阻（从43.5到5100 Ω）以上的内部电阻确定的极化曲线降低校准上限从240到30毫克/升O2 BOD 5。此外，更多的可发酵碳源增加了检测范围，如用真实的废水样品和仅含GGA、仅含葡萄糖和仅含谷氨酸的合成培养基所测试的。因此，生物膜驯化没有考虑到需氧需氧量测定和厌氧MFC响应之间的差异;这些可能是由于竞争过程，如发酵。这进一步突出了MFC作为实时传感器的潜力，除了BOD兼容的测量系统外，还可用于有机负荷监测和过程控制。在>800天内评估生物电化学传感器的校准，并且在独立重复的情况下保持稳定。动态生化需氧量的检测范围随外阻力的降低而增大。分析物组成影响传感器BOD 5预测，因此校准物的组成应与测量的分析物相似。
Microbial Fuel Cells (MFCs) operated as biosensors could potentially enable truly low-cost, real-time monitoring of organic loading in wastewaters. The current generated by MFCs has been correlated with conventional measures of organic load such as Biochemical Oxygen Demand (BOD), but much remains to be established in terms of the reliability and applicability of such sensors. In this study, batch-mode and multi-stage, flow-mode MFCs were operated for over 800 days and regularly re-calibrated with synthetic wastewater containing glucose and glutamic acid (GGA). BOD5 calibration curves were obtained by normalising the current measured as a percentage of maximum current. There was little drift between recalibrations and non-linear Hill models of the combined dataset had R2 of 88–95%, exhibiting a stable response over time and across devices. Nonetheless, factors which do affect calibration were also assessed. Increasing external resistance (from 43.5 to 5100 Ω) above the internal resistance determined by polarisation curve decreased the calibration upper limit from 240 to 30 mg/l O2 BOD5. Furthermore, more fermentable carbon sources increased the detection range, as tested with samples of real wastewater and synthetic media containing GGA, glucose-only and glutamic acid-only. Biofilm acclimatisation therefore did not account for differences between aerobic oxygen demand determinations and anaerobic MFC responses; these are likely attributable to competitive processes such as fermentation. This further highlights the potential for MFCs as real-time sensors for organic load monitoring and process control in addition to BOD-compliant measurement systems. The calibration of a bioelectrochemical sensor was assessed over >800 days and remained stable with independent replicates. The dynamic biochemical oxygen demand detection range was increased as external resistance was lowered. Analyte composition affected sensor BOD5 prediction thus calibrants should be similar in composition to the analyte measured.
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影响因子：
12.8
作者：
Di Lorenzo, Mirella;Curtis, Tom P.;Scott, Keith
通讯作者：
Scott, Keith
DOI：
10.1099/00207713-42-3-390
发表时间：
1992-07-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
OUATTARA, AS;TRAORE, AS;GARCIA, JL
通讯作者：
GARCIA, JL
影响因子：
2.7
作者：
Kim, BH;Chang, IS;Kim, HJ
通讯作者：
Kim, HJ
DOI：
10.3390/s16010035
发表时间：
2015-12-28
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Hsieh MC;Cheng CY;Liu MH;Chung YC
通讯作者：
Chung YC
影响因子：
5.2
作者：
Rotaru AE;Woodard TL;Nevin KP;Lovley DR
通讯作者：
Lovley DR