Nitric oxide as a retrograde messenger in the nucleus tractus solitarii of rats during hypoxia.
Nitric oxide as a retrograde messenger in the nucleus tractus solitarii of rats during hypoxia.
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一氧化氮作为缺氧期间大鼠孤束核中的逆行信使。
DOI：
10.1113/jphysiol.1995.sp020828
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Kunio Shirato
中科院分区：
文献类型：
--
作者：
Hiromasa Ogawa;Akiko Mizusawa;Yoshihiro Kikuchi;W. Hida;H. Miki;Kunio Shirato
1. 我们研究了一氧化氮（NO）在孤独束核（NTS）呼吸调节中的作用，其中L‐谷氨酸释放与外周化学受体激活相关，可调节缺氧通气反应。2. 实验在未麻醉的自由活动大鼠中进行。首先，研究了微注射硝普钠（SNP， NO供体）或NG -单甲基- L -精氨酸（L - NMMA， NO合成酶抑制剂）对NTS缺氧通气反应的影响。其次，使用体内微透析，在L‐NMMA存在的情况下，检测缺氧期间细胞外L‐谷氨酸的变化。第三，研究了L - NMMA对外源性L -谷氨酸增加通气的影响。此外，我们测量了缺氧时NTS细胞外L -瓜氨酸浓度的变化，以间接评估NO的形成，并检测了MK - 801（一种NMDA受体拮抗剂）对缺氧时L -瓜氨酸水平的影响。3. SNP增加了常氧和缺氧时的通气。在正常缺氧时，L - NMMA不改变通气或谷氨酸水平，但显著减弱缺氧通气反应和低氧时谷氨酸水平的增加。L - NMMA的抑制作用被L -精氨酸阻断。外源性L‐谷氨酸的通气增强作用被L‐NMMA减弱。L -瓜氨酸在缺氧时增加，MK - 801抑制了这种增加。4. 我们提供了第一个体内证据，证明在NTS中，NO作为逆行信使在L -谷氨酸释放正反馈系统中起作用，有助于在缺氧时增强通气。
1. We examined the role of nitric oxide (NO) in respiratory regulation in the nucleus tractus solitarii (NTS), where L‐glutamate release associated with peripheral chemoreceptor activation modulates the hypoxic ventilatory response. 2. Experiments were performed in unanaesthetized freely moving rats. First, the effects on the hypoxic ventilatory response of sodium nitroprusside (SNP, a NO donor) or NG‐monomethyl‐L‐arginine (L‐NMMA, a NO synthase inhibitor), microinjected into the NTS, were investigated. Second, using in vivo microdialysis, changes in extracellular L‐glutamate during hypoxia were examined in the presence of L‐NMMA. Third, the effect of L‐NMMA on ventilatory augmentation by exogenous L‐glutamate was examined. Furthermore, we measured extracellular L‐citrulline concentration changes during hypoxia in the NTS to assess NO formation indirectly and also examined the effect of MK‐801 (an NMDA receptor antagonist) on L‐citrulline levels during hypoxia. 3. SNP increased ventilation during both normoxia and hypoxia. L‐NMMA did not alter ventilation or L‐glutamate levels during normoxia but significantly attenuated the hypoxic ventilatory response and the increase in L‐glutamate during hypoxia. The inhibition by L‐NMMA was blocked by L‐arginine. The ventilatory augmentation by exogenous L‐glutamate was attenuated by L‐NMMA. L‐Citrulline increased during hypoxia, and this increase was inhibited by MK‐801. 4. We provide the first in vivo evidence that, in the NTS, NO works as a retrograde messenger in an L‐glutamate‐releasing positive feedback system contributing to the augmentation of ventilation during hypoxia.
DOI：
10.1073/pnas.88.24.11285
发表时间：
1991-12-01
影响因子：
11.1
作者：
ODELL, TJ;HAWKINS, RD;ARANCIO, O
通讯作者：
ARANCIO, O
DOI：
10.1073/pnas.86.22.9030
发表时间：
1989-11-01
影响因子：
11.1
作者：
BREDT, DS;SNYDER, SH
通讯作者：
SNYDER, SH
DOI：
10.1073/pnas.88.17.7797
发表时间：
1991-09-01
影响因子：
11.1
作者：
DAWSON, TM;BREDT, DS;SNYDER, SH
通讯作者：
SNYDER, SH