A comprehensive analysis of the germline and expressed TCR repertoire in White Peking duck.
A comprehensive analysis of the germline and expressed TCR repertoire in White Peking duck.
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白北京鸭种系及TCR表达谱的综合分析
DOI：
10.1038/srep41426
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发表时间：
2017-01-30
影响因子：
4.6
通讯作者：
Zhao Y
中科院分区：
文献类型：
--
作者：
Yang Z;Sun Y;Ma Y;Li Z;Zhao Y;Ren L;Han H;Jiang Y;Zhao Y
近年来，许多免疫相关基因在鸭子身上得到了广泛的研究，但对它们的TCR基因却知之甚少。在此，我们测定了北京白鸭的种系和TCR基因表达库。鸭TCRα/δ、TCRγ和非常规TCRδ2基因座的基因组结构与哺乳动物或鸡的同源基因座高度保守。相比之下，鸭的TCRβ基因座以一种不同寻常的模式组织，即（Vβ）n-Dβ-(Jβ)2-Cβ1-(Jβ)4-Cβ2，这与哺乳动物的串联排列簇或某些硬骨鱼的转座子组织不同。除编码免疫球蛋白结构域的第一个外显子外，两个Cβ的后续外显子在核苷酸序列和外显子结构上表现出显著的多样性。根据核苷酸序列的一致性，49个Vα、30个Vδ、13个Vβ和15个Vγ独特基因片段分别被划分为3个Vα、5个Vδ、4个Vβ和6个Vγ亚群。系统发育分析表明，除Vβ1、Vγ5和Vγ6外，鸭的大部分V亚群在鸡中有密切的同源物。所有cDNA克隆的编码接头显示用于增加连接多样性的保守机制。总的来说，这些数据提供了对脊椎动物tcr进化的见解，并提高了我们对鸟类免疫系统的理解。
Recently, many immune-related genes have been extensively studied in ducks, but relatively little is known about their TCR genes. Here, we determined the germline and expressed repertoire of TCR genes in White Peking duck. The genomic organization of the duck TCRα/δ, TCRγ and unconventional TCRδ2 loci are highly conserved with their counterparts in mammals or chickens. By contrast, the duck TCRβ locus is organized in an unusual pattern, (Vβ)n-Dβ-(Jβ)2-Cβ1-(Jβ)4-Cβ2, which differs from the tandem-aligned clusters in mammals or the translocon organization in some teleosts. Excluding the first exon encoding the immunoglobulin domain, the subsequent exons of the two Cβ show significant diversity in nucleotide sequence and exon structure. Based on the nucleotide sequence identity, 49 Vα, 30 Vδ, 13 Vβ and 15 Vγ unique gene segments are classified into 3 Vα, 5 Vδ, 4 Vβ and 6 Vγ subgroups, respectively. Phylogenetic analyses revealed that most duck V subgroups, excluding Vβ1, Vγ5 and Vγ6, have closely related orthologues in chicken. The coding joints of all cDNA clones demonstrate conserved mechanisms that are used to increase junctional diversity. Collectively, these data provide insight into the evolution of TCRs in vertebrates and improve our understanding of the avian immune system.
通过人类T淋巴细胞性病毒-I的体外转化，选择兔CD4-CD4-T-T细胞受体粉 - /三角洲细胞。
影响因子：
15.3
作者：
Sawasdikosol, S;Hague, B F;Zhao, T M;Bowers, F S;Simpson, R M;Robinson, M;Kindt, T J
通讯作者：
Kindt, T J