L-alpha-glycerophosphate and L-lactate electrodes based on the electrochemical "wiring" of oxidases.
L-alpha-glycerophosphate and L-lactate electrodes based on the electrochemical "wiring" of oxidases.
复制标题
基于氧化酶电化学“线路”的 L-α-甘油磷酸和 L-乳酸电极。
DOI：
10.1021/ac00033a009
复制
发表时间：
1992
影响因子：
7.4
通讯作者：
A. Heller
中科院分区：
文献类型：
--
作者：
I. Katakis;A. Heller
标题电极是通过将各自的FAD氧化酶与聚（乙烯基吡啶）聚合物共固定在固体电极表面上构建的，所述聚（乙烯基吡啶）聚合物是用溴乙胺和Os（bpy）2Cl 2 N-衍生的。氧化还原聚合物-酶水凝胶使用聚（乙二醇）二缩水甘油醚交联在电极表面上。与葡萄糖氧化酶的情况一样，氧化还原聚合物充当电子中继“导线”，将电子直接从酶的FADH 2中心转移到电极。这种转移与FADH 2被分子氧再氧化的自然过程竞争。这些电极的响应与气氛（N2或空气），pH值，和底物浓度的变化进行了测定。的电催化电流的pH值分布不同于游离酶的活性，表现出更广泛的最大值，转移到更高的pH值。观察到的灵敏度和线性范围分别为2 × 10（-2）A M-1 cm-2和2.7 mM（L-α-甘油磷酸盐）和0.3 A M-1 cm-2和0.2 mM（L-乳酸盐），可与2 × 10（-2）A M-1 cm-2和10 mM（葡萄糖）进行比较。所有电极的0-90%响应时间为1 s或更短。
The title electrodes were constructed by coimmobilizing the respective FAD oxidases on solid electrode surfaces with a poly(vinyl pyridine) polymer which was N-derivatized with bromoethylamine and Os(bpy)2Cl2. The redox-polymer-enzyme hydrogels were cross-linked on the electrode surface using poly(ethylene glycol) diglycidyl ether. As in the case of glucose oxidase, the redox polymer acts as an electron relaying "wire" transferring electrons directly from the enzymes' FADH2 centers to the electrode. This transfer competes with the natural process of reoxidation of FADH2 by molecular oxygen. The variation of the response of these electrodes with the atmosphere (N2 or air), pH, and substrate concentration was determined. The pH profile of the electrocatalytic current differs from that of the activity of the free enzymes, exhibiting a broader maximum, shifted to higher pH values. The observed sensitivities and linear ranges are respectively 2 x 10(-2) A M-1 cm-2 and 2.7 mM for L-alpha-glycerophosphate, and 0.3 A M-1 cm-2 and 0.2 mM for L-lactate that may be compared to 2 x 10(-2) A M-1 cm-2 and 10 mM for glucose. The 0-90% response time for all electrodes is 1 s or less.
烷基磺酸盐和硫酸盐的分离和间接检测。
影响因子：
1.3
作者：
Pietrzyk,DJ;Rigas,PG;Yuan,DX
通讯作者：
Yuan,DX
黄素蛋白乳酸氧化酶的氧化还原电位。
影响因子：
2.9
作者：
Stankovich,M;Fox,B
通讯作者：
Fox,B
屎链球菌L-α-甘油磷酸氧化酶的结构与机制研究。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Claiborne,A
通讯作者：
Claiborne,A