Biocompatible Fluorescent Nanodiamonds as Multifunctional Optical Probes for Latent Fingerprint Detection.
Biocompatible Fluorescent Nanodiamonds as Multifunctional Optical Probes for Latent Fingerprint Detection.
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生物相容性的荧光纳米座符作为潜在指纹检测的多功能光学探针。
DOI：
10.1021/acsami.9b19245
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发表时间：
2020-02-05
影响因子：
9.5
通讯作者：
Neuman KC
中科院分区：
文献类型：
--
作者：
Jung HS;Cho KJ;Ryu SJ;Takagi Y;Roche PA;Neuman KC
利用荧光纳米材料检测潜在指纹(LFP)的文献很多，因为荧光是最灵敏的检测方法之一。尽管已经开发了许多用于潜在指纹检测的荧光探针，但仍然存在许多挑战，包括用于显示LFP的纳米颗粒的低选择性、复杂的处理、高本底和毒性。在这项研究中，我们展示了用聚乙烯吡咯烷酮(PVP)包覆的荧光纳米钻石(FND)进行生物兼容、高效和低本底的LFP检测。PVP包被的FND(FND@PVP)在细胞水平上具有生物相容性：它们既不抑制细胞增殖，也不通过凋亡或其他细胞杀伤途径诱导细胞死亡。此外，它们不会在细胞内引发免疫反应。PVP涂层增强了FND与不同底物的物理结合，特别是由于PVP固有的两亲性，使FND@PVP有效地结合到指纹脊上。FND@PVP在几分钟内就会显示清晰、明确的脊线结构，以及第一、第二和第三级LFP细节。结合这种结合特异性和显著的光学特性FND@PVP，可以实现高对比度、高效率、高选择性、高灵敏度和低背景干扰的LPF检测。结果表明，利用FND@PVP纳米粒子的多色发射和双模成像的无背景成像在LFP的高分辨率成像方面具有巨大的潜力。
There is an immense literature on detection of latent fingerprints (LFPs) with fluorescent nanomaterials because fluorescence is one of the most sensitive detection methods. Although many fluorescent probes have been developed for latent fingerprint detection, many challenges remain, including the low selectivity, complicated processing, high background, and toxicity of nanoparticles used to visualize LFPs. In this study we demonstrate biocompatible, efficient, and low background LFP detection with polyvinylpyrrolidone (PVP) coated fluorescent nanodiamonds (FNDs). PVP coated FND (FND@PVP) are biocompatible at the cellular level: They neither inhibit cellar proliferation, nor induce cell death via apoptosis or other cell killing pathways. Moreover, they do not elicit an immune response in cells. PVP coating enhances the physical adhesion of FND to diverse substrates and in particular results in efficient binding of FND@PVP to fingerprint ridges due to the intrinsic amphiphilicity of PVP. Clear, well-defined ridge structures with first, second, and third level LFP details are revealed within minutes by FND@PVP. The combination of this binding specificity and the remarkable optical properties FND@PVP permits the detection of LPFs with high contrast, efficiency, selectivity, sensitivity, and reduced background interference. Our results demonstrate that background free imaging via multi-color emission and dual-modal imaging of FND@PVP nanoparticles has great potential for high resolution imaging of LFPs.
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影响因子：
3.9
作者：
ESUMI, K;OYAMA, M
通讯作者：
OYAMA, M
影响因子：
16.6
作者：
通讯作者：
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纳米颗粒与树突状细胞之间的相互作用：从癌症免疫治疗的角度
影响因子：
4.7
作者：
Jia J;Zhang Y;Xin Y;Jiang C;Yan B;Zhai S
通讯作者：
Zhai S
影响因子：
12.5
作者：
Doi, Y.;Makino, T.;Mizuochi, N.
通讯作者：
Mizuochi, N.
影响因子：
2.5
作者：
Abbas, Mohamed;Takahashi, Migaku;Kim, CheolGi
通讯作者：
Kim, CheolGi