In Vivo Extracellular pH Mapping of Tumors Using Electron Paramagnetic Resonance.
In Vivo Extracellular pH Mapping of Tumors Using Electron Paramagnetic Resonance.
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DOI：
10.1021/acs.analchem.8b03328
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发表时间：
2018-12-04
影响因子：
7.4
通讯作者：
Hirata H
中科院分区：
文献类型：
--
作者：
Komarov DA;Ichikawa Y;Yamamoto K;Stewart NJ;Matsumoto S;Yasui H;Kirilyuk IA;Khramtsov VV;Inanami O;Hirata H
建立了一种基于电子顺磁共振(EPR)的无创三维胞外pH定位方法，该方法以pH敏感的硝基自由基为外源顺磁探针。采用恒定磁场扫描的快速投影扫描能够在7.5分钟内获得四维(3D空间+1D频谱)EPR图像。通过对图像中pH依赖的光谱信息进行处理，重建了pH的三维图谱。为了验证所提出的pH定位方法，研究了荷瘤小鼠腿部细胞外酸中毒的进展。此外，细胞外pH图被用来显示不同的人源性胰腺导管癌细胞移植瘤小鼠模型中酸化的空间分布。所提出的基于EPR的pH图方法能够定量显示由于肿瘤代谢改变而引起的细胞外pH的变化。
An electron paramagnetic resonance (EPR) based method for noninvasive three-dimensional extracellular pH mapping was developed using a pH-sensitive nitroxyl radical as an exogenous paramagnetic probe. Fast projection scanning with a constant magnetic field sweep enabled the acquisition of four-dimensional (3D spatial + 1D spectral) EPR images within 7.5 min. Three-dimensional maps of pH were reconstructed by processing the pH-dependent spectral information of the images. To demonstrate the proposed method of pH mapping, the progress of extracellular acidosis in tumor-bearing mouse legs was studied. Furthermore, extracellular pH mapping was used to visualize the spatial distribution of acidification in different tumor xenograft mouse models of human-derived pancreatic ductal adenocarcinoma cells. The proposed EPR-based pH mapping method enabled quantitative visualization of changes in extracellular pH due to altered tumor metabolism.
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影响因子：
3.3
作者：
Foster, MA;Grigor'ev, IA;Nicholson, I
通讯作者：
Nicholson, I
影响因子：
--
作者：
GILLIES, RJ;LIU, Z;BHUJWALLA, Z
通讯作者：
BHUJWALLA, Z
影响因子：
3.1
作者：
Chen LQ;Randtke EA;Jones KM;Moon BF;Howison CM;Pagel MD
通讯作者：
Pagel MD
影响因子：
3.3
作者：
Bobko, Andrey A.;Eubank, Timothy D.;Voorhees, Jeffrey L.;Efimova, Olga V.;Kirilyuk, Igor A.;Petryakov, Sergey;Trofimiov, Dmitrii G.;Marsh, Clay B.;Zweier, Jay L.;Grigor'ev, Igor A.;Samouilov, Alexandre;Khramtsov, Valery V.
通讯作者：
Khramtsov, Valery V.
间质性无机磷酸盐作为肿瘤进展的肿瘤微环境标记。
影响因子：
4.6
作者：
Bobko AA;Eubank TD;Driesschaert B;Dhimitruka I;Evans J;Mohammad R;Tchekneva EE;Dikov MM;Khramtsov VV
通讯作者：
Khramtsov VV