Effects of Radiation on the Bone Strength of Spinal Vertebrae in Rats
Effects of Radiation on the Bone Strength of Spinal Vertebrae in Rats
复制标题
辐射对大鼠脊柱骨强度的影响
DOI：
10.1097/brs.0000000000004282
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Hiroyuki Tsuchiya
中科院分区：
文献类型：
--
作者：
Takashi Igarashi;Satoru Demura;Satoshi Kato;Kazuya Shinmura;Noriaki Yokogawa;Noritaka Yonezawa;Takaki Shimizu;Norihiro Oku;Hideki Murakami;Hiroyuki Tsuchiya
研究设计：一项对照实验室研究。目的：本研究的目的是检测放射对大鼠脊椎骨造成的骨损伤。背景资料概要：放射疗法广泛用于治疗恶性脊柱肿瘤。然而，少数研究报告放疗后椎体骨折为不良反应。目前还没有关于辐射引起的骨脆性变化、脊柱机械和结构变化以及辐射引起骨骨质疏松症的机制的报道。方法：将84只雌性Wistar大鼠随机分为20戈伊照射组和非照射组（对照组）。用20戈伊的外部局灶辐射剂量照射腰椎。在照射后0、2、4、6、8、12和24周进行生物力学、结构和组织学分析。用μCT进行椎体松质骨结构分析和骨密度测量。结果：照射后2周骨强度（311±23 N）较照射前（398±34 N）下降22%（P< 0.05）;与未照射组相比，照射组骨小梁间距增加，骨小梁连接性和宽度显著降低。照射后三维结构模型变得粗糙，骨小梁结构继续变薄和破坏。两组的骨密度无明显变化。结论：照射后2周观察到骨强度下降。骨矿物质密度保持不变，而骨小梁的微观结构发生了变化，提示骨损伤的辐射。证据等级：N/A我们检查了辐射对大鼠脊椎骨造成的损伤。照射后2周观察到骨强度下降。骨矿物质密度保持不变，而骨小梁的显微结构发生变化，表明骨损伤的辐射。
Study Design.A controlled laboratory study.Objective.The aim of this study was to examine bone damage caused by irradiation to spinal vertebrae in rats.Summary of Background Data.Radiotherapy is widely used in the treatment of malignant spine tumors. However, a few studies have reported vertebral fractures following radiotherapy as an adverse reaction. There are no reports on irradiation-induced changes in bone fragility, mechanical and structural changes focusing on the spine, and the mechanism of irradiation-induced bone osteoporosis.Methods.Eighty-four female Wistar rats were randomly allocated to the 20 Gy irradiated or the nonirradiated (control) group. The lumbar vertebrae were irradiated with an external focal radiation dose of 20 Gy. Biomechanical, structural, and histological analyses were performed at 0, 2, 4, 6, 8, 12, and 24 weeks after irradiation. Structural analysis and bone density measurement of vertebral trabecular bone were performed by μCT. Histopathological evaluation was performed by hematoxylin and eosin staining and immunostaining.Results.The bone strength at 2 weeks after irradiation (311±23 N) was 22% lower than that before irradiation (398±34 N)(P< 0.05). The trabecular spacing increased, and trabecular connectivity and width decreased significantly in the irradiated group compared with those in the non-irradiated group. The three-dimensional structure model became coarse, and the trabecular structure continued to thin and disrupt after irradiation. There was no significant change in the bone mineral density in both groups.Conclusion.A decrease in bone strength was observed 2 weeks after irradiation. Bone mineral density remained unaltered, whereas the microstructure of trabecular bone changed, suggesting bone damage by irradiation.Level of Evidence: N/AWe examined the damage caused by irradiation to spinal vertebrae in rats. Decrease in bone strength was observed 2 weeks after irradiation. Bone mineral density remained unaltered, whereas the microstructure of trabecular bone changed, suggesting bone damage by irradiation.
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影响因子：
2.8
作者：
Boehling, Nicholas S.;Grosshans, David R.;Chang, Eric L.
通讯作者：
Chang, Eric L.
DOI：
10.1016/0360-3016(93)90242-n
发表时间：
1993-09
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
Yeh-Chi Lo;Yeh-Chi Lo;Jeremy M.G. Taylor;William H. McBride;H. Withers
通讯作者：
Yeh-Chi Lo;Yeh-Chi Lo;Jeremy M.G. Taylor;William H. McBride;H. Withers
DOI：
10.1016/j.ijrobp.2012.04.034
发表时间：
2012-11-01
影响因子：
7
作者：
Cunha, Marcelo V. R.;Al-Omair, Ameen;Sahgal, Arjun
通讯作者：
Sahgal, Arjun
放疗后 25 年发生腰椎放射性骨坏死。
DOI：
10.1302/0301-620x.93b9.25991
发表时间：
2011
期刊：
The Journal of bone and joint surgery. British volume
影响因子：
--
作者：
D. Rolton;Stuart Blagg;R. Hughes
通讯作者：
R. Hughes
影响因子：
45.3
作者：
Rose, Peter S.;Laufer, Ilya;Lis, Eric
通讯作者：
Lis, Eric