Wheat VQ Motif-Containing Protein VQ25-A Facilitates Leaf Senescence via the Abscisic Acid Pathway.

Wheat VQ Motif-Containing Protein VQ25-A Facilitates Leaf Senescence via the Abscisic Acid Pathway.
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小麦VQ基序蛋白VQ25-A通过脱落酸途径促进叶片衰老。

DOI:
10.3390/ijms241813839
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发表时间:
2023-09-08
影响因子:
5.6
通讯作者:
--
中科院分区:
生物学2区
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叶片衰老是影响作物从养分同化向养分再动员功能转变的重要因素。小麦叶片的衰老对其产量和品质具有重要意义。在叶片衰老过程中,转录调控是整合各种衰老相关信号的重要环节。虽然已知植物特异性转录调节因子缬氨酸-谷氨酰胺(VQ)基因家族参与不同的生理过程,但其在叶片衰老中的作用尚不清楚。我们分离了TaVQ25-A并研究了其在叶片衰老调控中的作用。TaVQ25-A主要表达于小麦的根和叶中。TaVQ25-A-GFP融合蛋白定位于小麦原生质体的细胞核和细胞质中。tavq25 -A沉默的小麦植株在黑暗和ABA处理后出现延迟衰老表型。相反,过表达TaVQ25-A会加速叶片衰老,导致aba诱导的拟南芥叶片衰老超敏。WRKY型转录因子TaWRKY133与ABA通路密切相关,影响ABA相关基因的表达,在体外和体内均与TaVQ25-A相互作用。结果表明,TaVQ25-A是aba相关叶片衰老的正调控基因,可作为小麦分子育种的候选基因。
Leaf senescence is an important factor affecting the functional transition from nutrient assimilation to nutrient remobilization in crops. The senescence of wheat leaves is of great significance for its yield and quality. In the leaf senescence process, transcriptional regulation is a committed step in integrating various senescence-related signals. Although the plant-specific transcriptional regulation factor valine-glutamine (VQ) gene family is known to participate in different physiological processes, its role in leaf senescence is poorly understood. We isolated TaVQ25-A and studied its function in leaf senescence regulation. TaVQ25-A was mainly expressed in the roots and leaves of wheat. The TaVQ25-A-GFP fusion protein was localized in the nuclei and cytoplasm of wheat protoplasts. A delayed senescence phenotype was observed after dark and abscisic acid (ABA) treatment in TaVQ25-A-silenced wheat plants. Conversely, overexpression of TaVQ25-A accelerated leaf senescence and led to hypersensitivity in ABA-induced leaf senescence in Arabidopsis. A WRKY type transcription factor, TaWRKY133, which is tightly related to the ABA pathway and affects the expression of some ABA-related genes, was found to interact with TaVQ25-A both in vitro and in vivo. Results of this study indicate that TaVQ25-A is a positive regulator of ABA-related leaf senescence and can be used as a candidate gene for wheat molecular breeding.
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