Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate.
Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate.
复制标题
对乙酰甲胺磷不同敏感性的小菜蛾品系中乙酰胆碱酯酶基因的复制。
DOI：
10.1016/j.ibmb.2014.02.008
复制
发表时间：
2014
影响因子：
3.8
通讯作者：
T. Miyata
中科院分区：
文献类型：
--
作者：
S. Sonoda;Xueyan Shi;D. Song;P. Liang;Xiwu Gao;Youjun Zhang;Jianhong Li;Yong Liu;Ming Li;M. Matsumura;S. Sanada‐Morimura;C. Minakuchi;Toshiharu Tanaka;T. Miyata
本研究对乙酰甲胺磷敏感性不同的小菜蛾（Plutella xylostella）品系的乙酰胆碱酯酶1基因（AChE1）进行了分析。在野外收集的菌株中发现了该基因的多个单倍型，包括携带一个或两个先前报道的突变（A298S和G324A）的不同单倍型。此外，测序结果表明，在现场收集的菌株中存在重复的基因拷贝。AChE1拷贝数与乙酰甲胺磷抗性水平之间没有相关性，表明至少在某些小菜蛾品系中，AChE1的广泛复制不是主要的抗性因素。A298S和G324A突变的比例与乙酰甲胺磷抗性水平无关。这表明小菜蛾对乙酰甲胺磷的抗性是复杂的，不能仅根据AChE1拷贝数或抗性突变的比例来评估。
This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the field-collected strains. No correlation was found between copy numbers ofAChE1and levels of resistance to acephate suggesting that extensiveAChE1duplication is not a major resistance factor at least in someP. xylostellastrains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance ofP. xylostellais complex and cannot be evaluated based on theAChE1copy number or proportions of the resistance mutations alone.
影响因子：
1.3
作者：
Xiao-yu Ni;T. Tomita;S. Kasai;Y. Kono
通讯作者：
Xiao-yu Ni;T. Tomita;S. Kasai;Y. Kono
影响因子：
10.7
作者：
Tamura, Koichiro;Dudley, Joel;Kumar, Sudhir
通讯作者：
Kumar, Sudhir