Environmental Cues Contribute to Dynamic Plasma Membrane Organization of Nanodomains Containing Flotillin-1 and Hypersensitive Induced Reaction-1 Proteins in Arabidopsis thaliana.
Environmental Cues Contribute to Dynamic Plasma Membrane Organization of Nanodomains Containing Flotillin-1 and Hypersensitive Induced Reaction-1 Proteins in Arabidopsis thaliana.
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DOI：
10.3389/fpls.2022.897594
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发表时间：
2022
影响因子：
5.6
通讯作者：
Lin, Jinxing
中科院分区：
文献类型：
--
作者：
Xu, Changwen;Abbas, Sammar;Qian, Hongping;Yu, Meng;Zhang, Xi;Li, Xiaojuan;Cui, Yaning;Lin, Jinxing
关键词：
质膜是异质的并且含有多个功能纳米结构域。虽然几种信号蛋白已被证明通过移入或移出纳米结构域来发挥作用，但关于环境线索对纳米结构域组织的影响知之甚少。在这项研究中，我们研究了不同的纳米结构域的异质性和组织，包括那些含有拟南芥flotillin-1（AtFlot 1）和过敏诱导反应-1蛋白（AtHIR 1），在生物和非生物胁迫。可变角度全内反射荧光显微镜结合单粒子跟踪（SPT）显示，AtFlot 1和AtHIR 1表现出不同的横向动力学和栖息不同类型的纳米畴。此外，通过SPT和荧光相关光谱，我们观察到较低的密度和强度的AtFlot 1在质膜生物胁迫后的荧光。相反，AtHIR 1信号的密度和强度显着增加，响应于生物胁迫。在非生物胁迫下，AtFlot 1和AtHIR 1信号的密度和强度均显著降低。重要的是，SPT加上光漂白后的荧光恢复表明，生物和非生物胁迫可以调节AtFlot 1的动态，但是，只有非生物胁迫可以调节AtHIR 1的动态。总之，这些发现表明，大量的高度不同的纳米结构域共存于质膜（PM）中，不同的纳米结构域可以响应生物和非生物胁迫执行不同的功能。这些现象可以通过质膜蛋白与其相关的信号传导组分在专用PM纳米结构域内的空间聚集来解释。
Plasma membranes are heterogeneous and contain multiple functional nanodomains. Although several signaling proteins have been shown to function by moving into or out of nanodomains, little is known regarding the effects of environmental cues on nanodomain organization. In this study, we investigated the heterogeneity and organization of distinct nanodomains, including those containing Arabidopsis thaliana flotillin-1 (AtFlot1) and hypersensitive induced reaction-1 proteins (AtHIR1), in response to biotic and abiotic stress. Variable-angle total internal reflection fluorescence microscopy coupled with single-particle tracking (SPT) revealed that AtFlot1 and AtHIR1 exhibit different lateral dynamics and inhabit different types of nanodomains. Furthermore, via SPT and fluorescence correlation spectroscopy, we observed lower density and intensity of AtFlot1 fluorescence in the plasma membrane after biotic stress. In contrast, the density and intensity of signal indicating AtHIR1 markedly increased in response to biotic stress. In response to abiotic stress, the density and intensity of both AtFlot1 and AtHIR1 signals decreased significantly. Importantly, SPT coupled with fluorescence recovery after photobleaching revealed that biotic and abiotic stress can regulate the dynamics of AtFlot1; however, only the abiotic stress can regulate AtHIR1 dynamics. Taken together, these findings suggest that a plethora of highly distinct nanodomains coexist in the plasma membrane (PM) and that different nanodomains may perform distinct functions in response to biotic and abiotic stresses. These phenomena may be explained by the spatial clustering of plasma membrane proteins with their associated signaling components within dedicated PM nanodomains.
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影响因子：
7.4
作者：
Cui, Yaning;Zhao, Yanxia;Li, Xiaojuan
通讯作者：
Li, Xiaojuan
影响因子：
11.6
作者：
Majeran, Wojciech;Le Caer, Jean-Pierre;Giglione, Carmela
通讯作者：
Giglione, Carmela
影响因子：
16.6
作者：
Flores-Otero J;Ahn KH;Delgado-Peraza F;Mackie K;Kendall DA;Yudowski GA
通讯作者：
Yudowski GA
DOI：
10.1073/pnas.0910081107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Haney, Cara H.;Long, Sharon R.
通讯作者：
Long, Sharon R.
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影响因子：
4.6
作者：
Cui, Yaning;Li, Xiaojuan;Lin, Jinxing
通讯作者：
Lin, Jinxing