Phylogenetic analysis of eIF4E-family members.
Phylogenetic analysis of eIF4E-family members.
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DOI：
10.1186/1471-2148-5-48
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发表时间：
2005-09-28
影响因子：
3.4
通讯作者：
Jagus, R
中科院分区：
文献类型：
--
作者：
Joshi, B;Lee, K;Maeder, DL;Jagus, R
真核生物中的翻译起始涉及由翻译因子eIF 4 E控制的mRNA向核糖体的募集。eIF 4 E结合mRNA的5 '-m7 Gppp帽结构。与帽类似物结合的eIF 4 E的三维结构类似于“杯状手”，其中帽结构夹在两个保守的Trp残基（H. sapiens eIF4E）。第三个保守的Trp残基（H. sapiens eIF 4 E）识别帽结构的7-甲基部分。对GenBank NR和dbEST数据库的评估揭示，许多生物体编码许多与eIF 4 E具有同源性的蛋白。人们对这些结构相关的蛋白质之间的关系知之甚少。通过结合Genbank数据库中的序列数据，我们从230个物种中鉴定出编码411个eIF 4 E家族成员的序列。这些序列已存入互联网访问的数据库，设计用于eIF 4 E家族成员的序列比较。大多数成员可以分为三类之一。I类成员携带相当于H的Trp-43和Trp-56的Trp残基。sapiens eIF 4 E，并且似乎存在于所有真核生物中。II类成员，相对于H的Trp-43和Trp-56具有Trp→Tyr/Phe/Leu和Trp→Tyr/Phe取代。sapiens eIF 4 E，并且可以在后生动物、绿色植物和真菌中鉴定。III类成员具有相当于H的Trp-43的Trp残基。sapiens eIF 4 E，但相对于H. sapiens eIF 4 E，并且可以在腔肠动物和刺胞动物中鉴定。来自原生生物的一些eIF 4 E-家族成员显示相对于原型eIF 4 E-家族成员的延伸或压缩。扩增所有真核生物界的cDNA和基因组DNA序列，揭示了多种与eIF 4 E结构相关的蛋白质。从进化上看，似乎单个早期eIF 4 E基因经历了多个基因复制，产生了多个结构类别，因此不再可能从eIF 4 E家族成员的一级氨基酸序列预测功能。eIF 4 E家族成员的多样性为eIF 4 E结构主题提供了替代来源，这将有利于结构/功能分析和治疗药物设计。
Translation initiation in eukaryotes involves the recruitment of mRNA to the ribosome which is controlled by the translation factor eIF4E. eIF4E binds to the 5'-m7Gppp cap-structure of mRNA. Three dimensional structures of eIF4Es bound to cap-analogues resemble 'cupped-hands' in which the cap-structure is sandwiched between two conserved Trp residues (Trp-56 and Trp-102 of H. sapiens eIF4E). A third conserved Trp residue (Trp-166 of H. sapiens eIF4E) recognizes the 7-methyl moiety of the cap-structure. Assessment of GenBank NR and dbEST databases reveals that many organisms encode a number of proteins with homology to eIF4E. Little is understood about the relationships of these structurally related proteins to each other. By combining sequence data deposited in the Genbank databases, we have identified sequences encoding 411 eIF4E-family members from 230 species. These sequences have been deposited into an internet-accessible database designed for sequence comparisons of eIF4E-family members. Most members can be grouped into one of three classes. Class I members carry Trp residues equivalent to Trp-43 and Trp-56 of H. sapiens eIF4E and appear to be present in all eukaryotes. Class II members, possess Trp→Tyr/Phe/Leu and Trp→Tyr/Phe substitutions relative to Trp-43 and Trp-56 of H. sapiens eIF4E, and can be identified in Metazoa, Viridiplantae, and Fungi. Class III members possess a Trp residue equivalent to Trp-43 of H. sapiens eIF4E but carry a Trp→Cys/Tyr substitution relative to Trp-56 of H. sapiens eIF4E, and can be identified in Coelomata and Cnidaria. Some eIF4E-family members from Protista show extension or compaction relative to prototypical eIF4E-family members. The expansion of sequenced cDNAs and genomic DNAs from all eukaryotic kingdoms has revealed a variety of proteins related in structure to eIF4E. Evolutionarily it seems that a single early eIF4E gene has undergone multiple gene duplications generating multiple structural classes, such that it is no longer possible to predict function from the primary amino acid sequence of an eIF4E-family member. The variety of eIF4E-family members provides a source of alternatives on the eIF4E structural theme that will benefit structure/function analyses and therapeutic drug design.
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影响因子：
3.9
作者：
Douglas, SE;Penny, SL
通讯作者：
Penny, SL
DOI：
10.1083/jcb.150.2.f51
发表时间：
2000-07-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lasko P
通讯作者：
Lasko P
影响因子：
3.5
作者：
Aliyeva, E;Metz, AM;Browning, KS
通讯作者：
Browning, KS
影响因子：
5.3
作者：
Dinkova, TD;Keiper, BD;Rhoads, RE
通讯作者：
Rhoads, RE
影响因子：
4.8
作者：
Keiper, BD;Lamphear, BJ;Rhoads, RE
通讯作者：
Rhoads, RE