Effect of L-arginine supplement on liver regeneration after partial hepatectomy in rats.
Effect of L-arginine supplement on liver regeneration after partial hepatectomy in rats.
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DOI：
10.1186/1477-7819-10-99
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发表时间：
2012-05-31
影响因子：
3.2
通讯作者：
Sugiyama S
中科院分区：
文献类型：
--
作者：
Kurokawa T;An J;Tsunekawa K;Shimomura Y;Kazama S;Ishikawa N;Nonami T;Sugiyama S
据报道，一氧化氮（NO）是肝脏再生过程中肝细胞增殖的关键介质。 NO 是 L-精氨酸的氧化代谢产物，由一系列酶产生，统称为一氧化氮合酶 (NOS)。因此，给予 L-精氨酸可能会增强肝切除术后的肝再生。另一种氨基酸 L-谷氨酰胺在分解代谢状态中发挥着重要作用，并且是各种细胞和器官功能的关键因素，众所周知，它可以在实验中增强肝脏再生。因此，本研究旨在评估补充 L-精氨酸对肝再生的影响，并使用大鼠部分肝切除模型将其与补充 L-谷氨酰胺和 L-丙氨酸（后者作为阴性对照）进行比较。在 70% 肝切除术之前和之后，大鼠接受三种氨基酸溶液（L-精氨酸、L-谷氨酰胺或 L-丙氨酸）中的一种。通过评估术后24小时和72小时恢复的肝脏质量、增殖细胞核抗原(PCNA)染色以及总RNA和DNA含量来检查所施用的溶液对肝再生的影响。肝切除后72小时，L-精氨酸组和L-谷氨酰胺组恢复的肝脏质量、PCNA标记指数和DNA数量均显着高于对照组。 L-精氨酸组和L-谷氨酰胺组之间这些参数没有显着差异，L-丙氨酸组和对照组之间也没有发现任何显着差异。口服补充 L-精氨酸和 L-谷氨酰胺可增强大鼠肝切除术后的肝脏再生，这表明口服精氨酸补充剂可以在临床上改善主要肝脏切除术后的恢复。
Nitric oxide (NO) has been reported to be a key mediator in hepatocyte proliferation during liver regeneration. NO is the oxidative metabolite of L-arginine, and is produced by a family of enzymes, collective termed nitric oxide synthase (NOS). Thus, administration of L-arginine might enhance liver regeneration after a hepatectomy. Another amino acid, L-glutamine, which plays an important role in catabolic states and is a crucial factor in various cellular and organ functions, is widely known to enhance liver regeneration experimentally. Thus, the present study was undertaken to evaluate the effects of an L-arginine supplement on liver regeneration, and to compared this with supplementation with L-glutamine and L-alanine (the latter as a negative control), using a rat partial hepatectomy model. Before and after a 70% hepatectomy, rats received one of three amino acid solutions (L-arginine, L-glutamine, or L-alanine). The effects on liver regeneration of the administered solutions were examined by assessment of restituted liver mass, staining for proliferating cell nuclear antigen (PCNA), and total RNA and DNA content 24 and 72 hours after the operation. At 72 hours after the hepatectomy, the restituted liver mass, the PCNA labeling index and the DNA quantity were all significantly higher in the L-arginine and L-glutamine groups than in the control. There were no significant differences in those parameters between the L-arginine and L-glutamine groups, nor were any significant differences found between the L-alanine group and the control. Oral supplements of L-arginine and L-glutamine enhanced liver regeneration after hepatectomy in rats, suggesting that an oral arginine supplement can clinically improve recovery after a major liver resection.
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影响因子：
2.2
作者：
YOSHIDA, S;YUNOKI, T;KAKEGAWA, T
通讯作者：
KAKEGAWA, T
DOI：
10.1016/j.bbadis.2004.05.004
发表时间：
2004-09-06
影响因子：
6.2
作者：
Ronco, MT;Alvarez, MD;Carnovale, CE
通讯作者：
Carnovale, CE
影响因子：
7.3
作者：
Yao, J.;Zhu, Y.;Kitamura, M.
通讯作者：
Kitamura, M.
影响因子：
3.1
作者：
Assy, Nimer;Hussein, Osamah;Spira, Gadi
通讯作者：
Spira, Gadi
影响因子：
4.4
作者：
Mazza, RPD;Bertevello, PL;Waitzberg, DL
通讯作者：
Waitzberg, DL