Sex chromosome evolution in amniotes: applications for bacterial artificial chromosome libraries.

Sex chromosome evolution in amniotes: applications for bacterial artificial chromosome libraries.
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DOI:
10.1155/2011/132975
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发表时间:
2011
影响因子:
--
通讯作者:
Edwards SV
Edwards SV
中科院分区:
其他
文献类型:
--
作者:
Janes DE;Valenzuela N;Ezaz T;Amemiya C;Edwards SV

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羊膜中性染色体对之间的变异性代表着一种动态的历史。由于羊膜是从一个共同的祖先分化出来的,它们的性染色体对以及更广泛的性别决定机制都发生了可逆的频繁变化。这些变化已经通过使用许多工具和实验方法进行了研究和表征,但也许最有效的方法是应用细菌人工染色体(BAC)文库。单个细菌克隆携带来自目标物种的一个个体的100-200 kb的序列,可以通过筛选分离,定位到核型上,并进行测序。通过这些技术,研究人员已经确定了羊膜中性染色体内容和组织的不同和相似之处,并解决了关于过去变化的频率和方向的假设。在这里,我们回顾了羊水中性染色体进化的研究,以及BAC文库的出现对这一研究领域的影响。
Variability among sex chromosome pairs in amniotes denotes a dynamic history. Since amniotes diverged from a common ancestor, their sex chromosome pairs and, more broadly, sex-determining mechanisms have changed reversibly and frequently. These changes have been studied and characterized through the use of many tools and experimental approaches but perhaps most effectively through applications for bacterial artificial chromosome (BAC) libraries. Individual BAC clones carry 100–200 kb of sequence from one individual of a target species that can be isolated by screening, mapped onto karyotypes, and sequenced. With these techniques, researchers have identified differences and similarities in sex chromosome content and organization across amniotes and have addressed hypotheses regarding the frequency and direction of past changes. Here, we review studies of sex chromosome evolution in amniotes and the ways in which the field of research has been affected by the advent of BAC libraries.
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