Analysis of microspike movements on the neuronal growth cone
Analysis of microspike movements on the neuronal growth cone
复制标题
神经元生长锥上微刺运动的分析
作者：
D. Bray;K. Chapman
鸡感觉神经节神经元的生长锥在组织培养中，以60秒的时间间隔拍摄，因为他们先进的基层。微刺（或“丝状伪足”）的数量与它们出现的时间和位置、它们的伸长速率、它们的侧向运动、它们的寿命以及它们消失的位置和方式一起记录。所有的微刺都经历了伸展、侧向运动和缩短的循环。这些都是不规则的和不可预测的，但显示系统的差异，这取决于它们发生在生长锥的位置。在前沿的生长锥微刺扩展发生在最高频率和微刺缩短发生在最低频率;当后者发生在这个区域，它往往涉及的进步的边缘的细胞的形式的lamellipodium。微刺丢失最常发生在生长锥的基部，通常是通过微刺缩回到细胞中。在生长锥的不同区域的微尖峰的增益和损失的计算表明，它们经历了净逆行流，其速率与生长锥的向前推进。单个微刺也可以从生长锥向后移动到轴突（或“神经突”）上，这是最常发生在粘附基质上的事件。我们的观察结果支持微尖峰运动在生长锥的推进中的直接作用。轴突伸长的主要力量似乎是微刺的收缩，将生长锥的前缘向前拉。在生长锥的更近端和外围区域，微刺经历逆行扫描运动，随后缩回到细胞中，这也可能有助于生长锥的向前运动。我们解释这些运动所产生的流动肌动蛋白丝和相关的蛋白质被纳入微刺和片状伪足在生长锥的前缘，向后传递，并被沉积到富含肌动蛋白的膜相关的皮层的轴突圆柱体。
Growth cones of chick sensory ganglion neurons in tissue culture were photographed at 60-sec intervals as they advanced over the substratum. Numbers of microspikes (or “filopodia”) were recorded together with the time and position of their appearance, their rate of elongation, their lateral movements, their lifespan, and the position and manner of their disappearance. All microspikes go through cycles of extension, lateral movement, and shortening. These are irregular and unpredictable but show systematic differences depending on where on the growth cone they occur. At the leading edge of the growth cone microspike extension occurs at highest frequency and microspike shortening occurs at the lowest frequency; when the latter occurs in this region it often involves the advance of the margin of the cell in the form of a lamellipodium. Microspike loss occurs most often at the base of the growth cone, usually by the retraction of the microspike into the cell. Calculations of the gain and loss of microspikes at different regions of the growth cone show that they undergo a net retrograde flow, the rate of which is correlated with the forward advance of the growth cone. Individual microspikes can also move backward from the growth cone onto the axon (or “neurite”), an event that occurs most often on adhesive substrata. Our observations support a direct role of microspike movement in the advance of the growth cone. The primary force for axonal elongation appears to be the contraction of microspikes pulling the leading margin of the growth cone forward. At more proximal and peripheral regions of the growth cone, microspikes undergo a retrograde sweeping motion, followed by retraction into the cell, which may also contribute to the forward movement of the growth cone. We interpret these movements as arising from a flow of actin filaments and associated proteins which are incorporated into microspikes and lamellipodia at the leading edge of the growth cone, passing backward, and being deposited into the actin-rich membrane- associated cortex of the axonal cylinder.
肌动蛋白凝胶和皮质细胞质的结构。
DOI：
10.1101/sqb.1982.046.01.053
发表时间：
1982
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Stossel,TP;Hartwig,JH;Yin,HL;Zaner,KS;Stendahl,OI
通讯作者：
Stendahl,OI
神经元运动：面纱和微刺的超微结构与其运动活动相关。
影响因子：
4
作者：
Tosney,KW;Wessells,NK
通讯作者：
Wessells,NK