High mucosal levels of tumor necrosis factor alpha messenger RNA in AIDS-associated cytomegalovirus-induced esophagitis.
High mucosal levels of tumor necrosis factor alpha messenger RNA in AIDS-associated cytomegalovirus-induced esophagitis.
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艾滋病相关巨细胞病毒诱导的食管炎中肿瘤坏死因子 α 信使 RNA 的粘膜水平较高。
DOI：
10.1016/s0016-5085(98)70635-3
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发表时间：
1998
期刊：
影响因子：
29.4
通讯作者：
Smith,PD
中科院分区：
文献类型：
--
作者：
Wilcox,CM;Harris,PR;Redman,TK;Kawabata,S;Hiroi,T;Kiyono,H;Smith,PD
背景与目的为了评价肿瘤坏死因子α（TNF-α）在巨细胞病毒相关性胃肠道疾病中的作用，我们定量测定了巨细胞病毒相关性食管炎和获得性免疫缺陷综合征患者食管黏膜中TNF-α mRNA的水平。通过原位杂交、逆转录聚合酶链反应和定量聚合酶链反应对编码食管标本中TNF-α mRNA的水平进行了评估。结果3例溃疡愈合或明显改善的患者的食管粘膜在治疗前含有大量表达TNF-α mRNA的巨噬细胞，并且治疗后TNF-α mRNA的组织水平大幅下降或检测不到。相比之下，治疗后溃疡恶化的单个患者的食管标本在治疗前和治疗后都含有许多表达TNF-α mRNA的粘膜巨噬细胞，结论巨噬细胞产生增加和TNF-α mRNA的高组织水平与巨细胞病毒感染有关，相关的食管溃疡，并可能有助于与巨细胞病毒诱导的胃肠道疾病相关的炎症反应。《胃肠病学》1998;114：77-82
Background & AimsTo evaluate the role of tumor necrosis factor α (TNF-α), a key inflammatory cytokine, in cytomegalovirus-associated gastrointestinal disease, we quantitated the level of TNF-α messenger RNA (mRNA) in esophageal mucosa from patients with cytomegalovirus-associated esophagitis and acquired immunodeficiency syndrome.MethodsFour patients underwent endoscopic biopsy of their cytomegalovirus-associated esophageal ulcers before and after ganciclovir therapy. The level of TNF-α mRNA in coded esophageal specimens was assessed by in situ hybridization, reverse-transcription polymerase chain reaction, and quantitative polymerase chain reaction.ResultsEsophageal mucosa from 3 patients whose ulcers healed or markedly improved contained before therapy numerous macrophages expressing TNF-α mRNA and high tissue levels of TNF-α mRNA that decreased substantially or were not detectable after therapy. In contrast, esophageal specimens from the single patient whose ulcer worsened after therapy contained many mucosal macrophages expressing TNF-α mRNA before as well as after therapy, and the high number of molecules of TNF-α mRNA present in the tissue before therapy increased further after treatment.ConclusionsIncreased macrophage production and high tissue levels of TNF-α mRNA are associated with cytomegalovirus-associated esophageal ulcers and probably contribute to the inflammatory response associated with cytomegalovirus-induced gastrointestinal disease. GASTROENTEROLOGY 1998;114:77-82
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影响因子：
3.3
作者：
SCHWARTZ, DA;WILCOX, CM
通讯作者：
WILCOX, CM
影响因子：
5.9
作者：
WILCOX, CM;STRAUB, RF;SCHWARTZ, DA
通讯作者：
SCHWARTZ, DA
HIV 相关腹泻患者粪便肿瘤坏死因子-α
影响因子：
3.8
作者：
D. Sharpstone;A. Rowbottom;M. Nelson;M. W. Lepper;B. Gazzard
通讯作者：
B. Gazzard
艾滋病中的食管疾病与病理过程有关，而不是与 1 型粘膜人类免疫缺陷病毒有关。
影响因子：
6.4
作者：
Phillip D. Smith;Mark S. Eisner;J. Manischewitz;Vee J. Gill;Henry Masur;Cecil F. Fox
通讯作者：
Cecil F. Fox
影响因子：
4.4
作者：
B. Beutler;I. Milsark;A. Cerami
通讯作者：
B. Beutler;I. Milsark;A. Cerami