Characterization of time-course morphological features for efficient prediction of osteogenic potential in human mesenchymal stem cells.
Characterization of time-course morphological features for efficient prediction of osteogenic potential in human mesenchymal stem cells.
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DOI：
10.1002/bit.25189
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发表时间：
2014-07
影响因子：
3.8
通讯作者：
Kato, Ryuji
中科院分区：
文献类型：
--
作者：
Matsuoka, Fumiko;Takeuchi, Ichiro;Agata, Hideki;Kagami, Hideaki;Shiono, Hirofumi;Kiyota, Yasujiro;Honda, Hiroyuki;Kato, Ryuji
关键词：
人骨髓间充质干细胞(HBMSCs)是临床骨再生最常用的细胞来源之一。为了达到最大的治疗效果，在干细胞植入前的培养过程中评估其成骨分化潜能是至关重要的。然而，干细胞成骨能力的实际评估仅限于侵入性生物标记物分析，仅能分析单个终点。为了在临床细胞治疗中围绕侵入性质量评估进行创新，我们先前探索并证明了使用分化培养过程中拍摄的时间进程图像对hBMSC骨分化潜力的积极预测价值。这种最初的方法为基于形态的细胞评估方法建立了概念证明，但在考虑处理大量图像数据的需要时，揭示了实际的局限性。在这份报告中，我们的目标是将我们提出的方法缩减为更实用、更有效的建模方案，以便医生在临床细胞治疗的前沿领域更广泛地实施。我们研究了在成骨分化过程中哪些形态特征是关键的，以确保预测模型的性能，同时减轻了图像采集的负担。据我们所知，这是第一次详细描述了形态特征充分模拟成骨潜力所需的关键观察期和关键时间点数量。我们的结果揭示了三个重要的观察结果：(I)分化前3天的形态特征足以预测连续培养3周后的骨分化潜能，碱性磷酸酶活性和钙沉积；(Ii)48 h的间隔足以测量关键的形态特征；(Iii)当分化前3天的早期形态特征与后来的特征(分化后10天)相结合时，形态特征是最准确的预测。生物技术。比昂斯。2014；111：1430-1439。
Human bone marrow mesenchymal stem cells (hBMSCs) represents one of the most frequently applied cell sources for clinical bone regeneration. To achieve the greatest therapeutic effect, it is crucial to evaluate the osteogenic differentiation potential of the stem cells during their culture before the implantation. However, the practical evaluation of stem cell osteogenicity has been limited to invasive biological marker analysis that only enables assaying a single end-point. To innovate around invasive quality assessments in clinical cell therapy, we previously explored and demonstrated the positive predictive value of using time-course images taken during differentiation culture for hBMSC bone differentiation potential. This initial method establishes proof of concept for a morphology-based cell evaluation approach, but reveals a practical limitation when considering the need to handle large amounts of image data. In this report, we aimed to scale-down our proposed method into a more practical, efficient modeling scheme that can be more broadly implemented by physicians on the frontiers of clinical cell therapy. We investigated which morphological features are critical during the osteogenic differentiation period to assure the performance of prediction models with reduced burden on image acquisition. To our knowledge, this is the first detailed characterization that describes both the critical observation period and the critical number of time-points needed for morphological features to adequately model osteogenic potential. Our results revealed three important observations: (i) the morphological features from the first 3 days of differentiation are sufficiently informative to predict bone differentiation potential, both activities of alkaline phosphatase and calcium deposition, after 3 weeks of continuous culture; (ii) intervals of 48 h are sufficient for measuring critical morphological features; and (iii) morphological features are most accurately predictive when early morphological features from the first 3 days of differentiation are combined with later features (after 10 days of differentiation). Biotechnol. Bioeng. 2014;111: 1430–1439.
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影响因子：
2.7
作者：
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通讯作者：
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影响因子：
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作者：
Matsuoka F;Takeuchi I;Agata H;Kagami H;Shiono H;Kiyota Y;Honda H;Kato R
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