Effect of GA3 treatment on seed development and seed-related gene expression in grape.
Effect of GA3 treatment on seed development and seed-related gene expression in grape.
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DOI：
10.1371/journal.pone.0080044
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Cheng C;Xu X;Singer SD;Li J;Zhang H;Gao M;Wang L;Song J;Wang X
植物激素赤霉酸（GA3）被广泛用于鲜食葡萄工业中以诱导种子品种的无核。然而，有一个缺乏的信息有关的机制，其中气体诱导无核葡萄。为了系统地分析这种GA 3诱导的种子败育的原因，我们对两个有籽葡萄品种（“巨峰”和“红地球”），沿着无核品种（“汤普森无核”），用GA 3处理后进行了深入的表征。以类似的方式无籽对照，表现出GA 3诱导的种子败育后9天盛开（ESTA），“巨峰”和“红地球仪”种子品种表现出完全败育的种子15天后，用GA 3处理。形态学分析表明，虽然受精似乎正常发生后，GA3处理，以及在未经处理的无核对照品种，种子生长最终停止。此外，我们发现GA3的应用对氧化还原平衡有影响，这可能会导致细胞损伤和随后的种子败育。此外，我们进行了抗氧化酶活性的分析，以及从各种基因的转录水平被认为是参与种子发育，并发现几个GA3处理和未处理的控制之间的差异。因此，看来，驱动GA 3诱导无核的机制是相似的，在有籽和无核品种，观察到的种子败育可能导致至少部分从GA 3诱导的活性氧引起的细胞损伤的增加，抗氧化酶活性的降低，以及与种子发育相关的基因的表达的改变。
The phytohormone gibberellic acid (GA3) is widely used in the table grape industry to induce seedlessness in seeded varieties. However, there is a paucity of information concerning the mechanisms by which GAs induce seedlessness in grapes. In an effort to systematically analyze the cause of this GA3-induced seed abortion, we conducted an in depth characterization of two seeded grape cultivars (‘Kyoho’ and ‘Red Globe’), along with a seedless cultivar (‘Thompson Seedless’), following treatment with GA3. In a similar fashion to the seedless control, which exhibited GA3-induced abortion of the seeds 9 days after full bloom (DAF), both ‘Kyoho’ and ‘Red Globe’ seeded varieties exhibited complete abortion of the seeds 15 DAF when treated with GA3. Morphological analyses indicated that while fertilization appeared to occur normally following GA3 treatment, as well as in the untreated seedless control cultivar, seed growth eventually ceased. In addition, we found that GA3 application had an effect on redox homeostasis, which could potentially cause cell damage and subsequent seed abortion. Furthermore, we carried out an analysis of antioxidant enzyme activities, as well as transcript levels from various genes believed to be involved in seed development, and found several differences between GA3-treated and untreated controls. Therefore, it seems that the mechanisms driving GA3-induced seedlessness are similar in both seeded and seedless cultivars, and that the observed abortion of seeds may result at least in part from a GA3-induced increase in cell damage caused by reactive oxygen species, a decrease in antioxidant enzymatic activities, and an alteration of the expression of genes related to seed development.
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影响因子：
5.1
作者：
Aubert, D;Chevillard, M;Herzog, M
通讯作者：
Herzog, M
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11.6
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Brambilla, Vittoria;Battaglia, Raffaella;Colombo, Lucia
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56.9
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Grossniklaus, U;Vielle-Calzada, JP;Gagliano, WB
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Gagliano, WB
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11.6
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