Enriched environment housing improved the laying hen's resistance to transport stress via modulating the heat shock protective response and inflammation.
Enriched environment housing improved the laying hen's resistance to transport stress via modulating the heat shock protective response and inflammation.
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丰富的环境住房通过调节热休克保护反应和炎症提高了蛋鸡对运输应激的抵抗力
DOI：
10.1016/j.psj.2020.12.036
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发表时间：
2021-03
期刊：
影响因子：
4.4
通讯作者：
Bao J
中科院分区：
文献类型：
--
作者：
Li C;Zhang R;Wei H;Wang Y;Chen Y;Zhang H;Li X;Liu H;Li J;Bao J
丰富的环境可以促进动物科普复杂环境的能力。将216只18周龄产蛋鸡随机分为2组，其中CC组36只，FC组180只。在64周龄时，每组选择24只鸡进行4小时的运输处理。分别于运输前、运输后即刻和运输后48 h采集蛋鸡脾脏组织，检测热休克保护反应信号通路和炎症因子的表达。收集血清标本检测免疫细胞因子的含量。运输应激可使CC组热休克蛋白（HSP;包括Small HSP、HSP 27、HSP 40、HSP 60、HSP 70、HSP 90、HSP 110）表达降低（P < 0.05），而FC组运输应激后即刻HSP（除Small HSP和HSP 40外）表达无显著性差异（P > 0.05）。运输后48 h，FC组HSP（除SmallHSP和HSP 40外）mRNA表达均高于BT组（P < 0.05）。HSP 60、HSP 70和HSP 90蛋白水平的变化趋势相似。结果表明，设施笼养可缓解运输应激对母鸡脾脏HSP表达的抑制作用。此外，FC组鸡的促炎因子（核转录因子-κ B、诱导型一氧化氮合酶、环氧合酶-2、前列腺素E合酶、炎性细胞因子[IL-1β和IL-6]和肿瘤坏死因子α）表达水平较低（P < 0.05）。提示富营养化环境可通过调节热休克反应蛋白和炎性细胞因子的表达，减轻蛋鸡运输应激损伤，提高蛋鸡对运输应激的抵抗力。
An enriched environment can promote adaptability of animals to cope with complex environments. A total of 18-week-old 216 laying hens were randomly divided into 2 groups; of which, one group was housed in conventional battery cages (CC, n = 36), and the others were housed in furnished cages (FC, n = 180). At the end of 64 wk of age, 24 chickens of each group were selected for 4-hour transport treatment. The spleen tissues of laying hens were collected before transportation (BT), immediately after transportation, and at 48 h after transportation to detect the expression of the heat shock protective response signaling pathway and inflammatory factors. Serum samples were collected to detect the content of immune cytokines. Transport stress decreased heat shock proteins (HSP; including Small HSP, HSP27, HSP40, HSP60, HS70, HSP90, HSP110) in the CC group (P < 0.05), whereas there was no significant difference in the expression of HSP (except for Small HSP and HSP40) in the FC group (P > 0.05) immediately after transportation. At 48 h after transportation, mRNA levels of HSP (except for Small HSP and HSP40) in the FC group were upregulated, which were higher than those at BT (P < 0.05). The changes in HSP60, HSP70, and HSP90 protein levels had similar tendencies. The results showed that housing in furnished cages alleviated the inhibition of expression of HSP in the hens' spleen induced by transport stress. In addition, the hens housed in the FC group had lower expression levels of proinflammatory factors (nuclear transcription factor-kappa B, inducible nitric oxide synthase, cyclooxygenase-2, prostaglandin E synthase, inflammatory cytokines [IL-1β and IL-6], and tumor necrosis factor alpha) (P < 0.05). We suggest that the enriched environment can reduce transport stress damage in laying hens and improve resistance to transport stress by regulating expression of heat shock response proteins and inflammatory cytokines.
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影响因子：
3.3
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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