Constrained tibial vibration in mice: a method for studying the effects of vibrational loading of bone.
Constrained tibial vibration in mice: a method for studying the effects of vibrational loading of bone.
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小鼠的受限胫骨振动：一种研究骨振动载荷影响的方法。
DOI：
10.1115/1.2917435
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发表时间：
2008-08
影响因子：
1.7
通讯作者：
Silva, Matthew J.
中科院分区：
文献类型：
--
作者：
Christiansen, Blaine A.;Bayly, Philip V.;Silva, Matthew J.
振动负荷可以刺激新骨小梁的形成，或维持骨量。研究振动载荷的研究经常使用全身振动（WBV）作为载荷方法。然而，WBV 在小动物研究中存在局限性，因为振动的传递能力取决于姿势。在这项研究中，我们提出约束胫骨振动（CTV）作为在受控条件下小鼠振动负载的实验方法。在 CTV 中，麻醉小鼠的小腿在支撑质量的同时承受垂直振动载荷。该设置近似于一个单自由度质量弹簧系统，其中腿充当弹簧。加速度计用于测量 CTV 中小腿（脚到膝盖）的振动传递率，频率为 20-150 Hz。首先，在体内量化传递性的频率响应，并进行解剖以去除小鼠小腿的一个组成部分（膝关节、足部或软组织），以研究每个组成部分对完整腿的频率响应的贡献。接下来，使用小鼠胫骨-腓骨的有限元模型来估计 CTV 期间骨骼的变形。最后，应变计用于确定骨应变对加载频率的依赖性。 CTV 系统中的活体小鼠腿的共振频率约为 60 Hz，振动质量为 125 g，振动为 ±0.5 G（峰峰值为 1.0 G）。移除脚导致系统的固有频率从 60 赫兹变为 70 赫兹；去除软组织不会导致固有频率发生变化；移除膝盖将固有频率从 60 赫兹更改为 90 赫兹。使用有限元模型，估计 CTV 期间的最大拉伸应变和压缩应变分别位于胫骨的颅内侧和尾外侧表面，并且峰值传递率和峰值皮质应变发生在相同的频率。应变计数据证实了 FE 模型所示的峰值传递率和峰值骨应变之间的关系，并表明完整腿 CTV 期间的最大循环胫骨应变为 330 ± 82 με，发生在 60–70 Hz 时。本研究提出了 CTV 的综合力学分析，这是一种研究受控条件下振动载荷的载荷方法。该模型将用于未来的体内研究，并有可能成为了解骨骼对振动载荷响应的重要工具。
Vibrational loading can stimulate the formation of new trabecular bone, or maintain bone mass. Studies investigating vibrational loading have often used whole-body vibration (WBV) as their loading method. However, WBV has limitations in small animal studies because transmissibility of vibration is dependent on posture. In this study we propose constrained tibial vibration (CTV) as an experimental method for vibrational loading of mice under controlled conditions. In CTV the lower leg of an anesthetized mouse is subjected to vertical vibrational loading while supporting a mass. The setup approximates a one degree-of-freedom mass-spring system where the leg acts as the spring. Accelerometers were used to measure the transmissibility of vibration through the lower leg (foot to knee) in CTV at frequencies from 20–150 Hz. First, the frequency response of transmissibility was quantified in vivo, and dissections were performed to remove one component of the mouse lower leg (the knee joint, foot, or soft tissue) in order to investigate the contribution of each component to the frequency response of the intact leg. Next, a finite element model of a mouse tibia-fibula was used to estimate the deformation of the bone during CTV. Finally, strain gages were used to determine the dependence of bone strain on loading frequency. The in vivo mouse leg in the CTV system had a resonant frequency at approximately 60 Hz for ±0.5 G vibration (1.0 G peak-to-peak) with a moving mass of 125 g. Removing the foot caused the natural frequency of the system to shift from 60 to 70 Hz; removing the soft tissue caused no change in natural frequency; and removing the knee changed the natural frequency from 60 to 90 Hz. Using the FE model, maximum tensile and compressive strains during CTV were estimated to be on the cranial-medial and caudo-lateral surfaces of the tibia, respectively, and the peak transmissibility and peak cortical strain occurred at the same frequency. Strain gage data confirmed the relationship between peak transmissibility and peak bone strain indicated by the FE model, and showed that the maximum cyclic tibial strain during CTV of the intact leg was 330 ± 82 με and occurred at 60–70 Hz. This study presents a comprehensive mechanical analysis of CTV, a loading method for studying vibrational loading under controlled conditions. This model will be used in future in vivo studies, and has the potential to become an important tool for understanding the response of bone to vibrational loading.
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影响因子：
6.2
作者：
Verschueren, SMP;Roelants, M;Boonen, S
通讯作者：
Boonen, S
影响因子：
6.2
作者：
Ward, K;Alsop, C;Mughal, Z
通讯作者：
Mughal, Z
影响因子：
6.2
作者：
Rubin, C;Turner, AS;Qin, YX
通讯作者：
Qin, YX
影响因子：
64.8
作者：
Rubin, C;Turner, AS;McLeod, K
通讯作者：
McLeod, K
DOI：
10.1002/ar.a.20171
发表时间：
2005-04-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Silva, MJ;Brodt, MD;Hucker, WJ
通讯作者：
Hucker, WJ