Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae.
Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae.
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Pep3p/Pep5p 复合物：囊泡运输到酿酒酵母液泡的多个步骤中的假定对接因子。
DOI：
10.1093/genetics/156.1.105
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Jones,EW
中科院分区：
文献类型：
--
作者：
Srivastava,A;Woolford,CA;Jones,EW
已知 Pep3p 和 Pep5p 对于将水解酶前体运输到液泡和液泡生物发生是必需的。这些蛋白质存在于介导液泡膜转运的异源寡聚复合物中。PEP5 与 VPS8 发生遗传相互作用，表明 Pep5p 参与早期高尔基体到内体的步骤和/或从内体到高尔基体的再循环。为了进一步了解 Pep3p 和 Pep5p 的细胞作用，我们分离并表征了一组 pep3 条件突变体。突变体的表征表明，pep3t 突变体在内体和非内体高尔基体到液泡运输途径、细胞质到液泡靶向途径、从内体循环回到晚期高尔基体以及内吞作用方面存在缺陷。PEP3 与内体 SNARE 复合体的两个成员 PEP12（t-SNARE）和 PEP7（哺乳动物同源物）在遗传上相互作用欧洲经济区1)；两种杂交分析表明，Pep3p 和 Pep5p 与 Pep7p 存在物理关联。我们的结果表明，核心 Pep3p/Pep5p 复合物在通往液泡的运输途径的多个步骤中与 SNARE 蛋白一起促进囊泡对接/融合反应。我们认为该复合物可能负责将运输囊泡束缚在目标膜上。
Pep3p and Pep5p are known to be necessary for trafficking of hydrolase precursors to the vacuole and for vacuolar biogenesis. These proteins are present in a hetero-oligomeric complex that mediates transport at the vacuolar membrane.PEP5interacts genetically withVPS8, implicating Pep5p in the earlier Golgi to endosome step and/or in recycling from the endosome to the Golgi. To understand further the cellular roles of Pep3p and Pep5p, we isolated and characterized a set ofpep3conditional mutants. Characterization of mutants revealed thatpep3tsmutants are defective in the endosomal and nonendosomal Golgi to vacuole transport pathways, in the cytoplasm to vacuole targeting pathway, in recycling from the endosome back to the late Golgi, and in endocytosis.PEP3interacts genetically with two members of the endosomal SNARE complex,PEP12(t-SNARE) andPEP7(homologue of mammalian EEA1); Pep3p and Pep5p associate physically with Pep7p as revealed by two-hybrid analysis. Our results suggest that a core Pep3p/Pep5p complex promotes vesicular docking/fusion reactions in conjunction with SNARE proteins at multiple steps in transport routes to the vacuole. We propose that this complex may be responsible for tethering transport vesicles on target membranes.
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Pep7p 提供了一种新型蛋白质，可在酵母高尔基体和内体之间的囊泡介导的运输中发挥作用。
影响因子：
3.3
作者：
Webb,GC;Zhang,J;Garlow,SJ;Wesp,A;Riezman,H;Jones,EW
通讯作者：
Jones,EW
影响因子：
3.3
作者：
P. James;J. Halladay;E. Craig
通讯作者：
P. James;J. Halladay;E. Craig
酿酒酵母的蛋白酶突变体。
影响因子：
3.3
作者：
E. W. Jones
通讯作者：
E. W. Jones
影响因子：
16
作者：
Sevrioukov, EA;He, JP;Krämer, H
通讯作者：
Krämer, H