Osteoblast maturation on microtextured titanium involves paracrine regulation of bone morphogenetic protein signaling.
Osteoblast maturation on microtextured titanium involves paracrine regulation of bone morphogenetic protein signaling.
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DOI：
10.1002/jbm.a.35308
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发表时间：
2015-05
影响因子：
4.9
通讯作者：
Boyan, Barbara D.
中科院分区：
文献类型：
--
作者：
Olivares-Navarrete, Rene;Hyzy, Sharon L.;Pan, Qingfen;Dunn, Ginger;Williams, Joseph K.;Schwartz, Zvi;Boyan, Barbara D.
成骨细胞对表面微形态和化学成分非常敏感。与光滑表面相比，微结构钛在体外具有更高的成骨细胞分化和成熟度，在体内促进骨形成和骨整合。细胞在微结构钛合金表面的BMP2表达增加，提示旁分泌在调节成骨细胞成熟中的作用。然而，最近的研究表明，外源性BMP2抑制成骨细胞产生抗炎细胞因子和骨钙素，提示可能参与了BMP信号转导的调控。这项研究检测了成骨细胞在钛表面成熟过程中，细胞是否调节BMP配体、受体和抑制物，特别是BMP2和Noggin的作用。与光滑表面相比，BMP2、BMP4和BMP7及其受体BMPR1A、BMPR1B和BMPR2的mRNA和蛋白以及BMP抑制物在微纹理表面上调。外源性BMP2对微结构钛的成熟度有轻微的促进作用，而Noggin的加入则抑制了成骨细胞的成熟。含Noggin的细胞被击倒，成骨细胞成熟度明显增加。这些结果表明，BMP相关分子在微结构钛表面的成骨细胞成熟过程中受到控制，内源性Noggin是这一过程的重要调节因子。与应用外源性BMP相比，修改旁分泌BMP信号可能产生更强健的骨形成。
Osteoblasts are sensitive to surface microtopography and chemistry. Osteoblast differentiation and maturation are higher in vitro and bone formation and osseointegration enhanced in vivo on microstructured titanium (Ti) compared to smooth surfaces. Cells increased BMP2 expression on microtextured Ti alloy, suggesting a paracrine role in regulating osteoblast maturation. However, recent studies show that exogenous BMP2 inhibits osteoblast production of anti-inflammatory cytokines and osteocalcin, indicating that control of BMP-signaling may be involved. This study examined whether cells modulate BMP ligands, receptors, and inhibitors during osteoblast maturation on Ti, specifically focusing on the roles of BMP2 and Noggin. mRNA and protein for BMP2, BMP4, and BMP7 and receptors BMPR1A, BMPR1B, and BMPR2, and BMP inhibitors were upregulated on microtextured surfaces in comparison to smooth surfaces. Maturation on microstructured Ti was slightly enhanced with exogenous BMP2 while Noggin addition inhibited osteoblast maturation. Cells with Noggin knocked down significantly increased osteoblast maturation. These results demonstrate that BMP-related molecules are controlled during osteoblast maturation on microstructured Ti surfaces and that endogenous Noggin is an important regulator of the process. Modifying paracrine BMP signaling may yield more robust bone formation than application of exogenous BMPs.
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影响因子：
5.4
作者：
Heinecke K;Seher A;Schmitz W;Mueller TD;Sebald W;Nickel J
通讯作者：
Nickel J
影响因子：
4
作者：
Hyzy, Sharon L.;Olivares-Navarrete, Rene;Schwartz, Zvi;Boyan, Barbara D.
通讯作者：
Boyan, Barbara D.
影响因子：
64.8
作者：
MATZUK, MM;LU, NF;BRADLEY, A
通讯作者：
BRADLEY, A
影响因子：
4.3
作者：
Donos, N.;Hamlet, S.;Ivanovski, S.
通讯作者：
Ivanovski, S.
影响因子：
2.7
作者：
Baek JA;Lan Y;Liu H;Maltby KM;Mishina Y;Jiang R
通讯作者：
Jiang R