The human myeloma cell line LP-1: a versatile model in which to study early plasma-cell differentiation and c-myc activation.-猫眼课题宝

人骨髓瘤细胞系 LP-1：一种用于研究早期浆细胞分化和 c-myc 激活的多功能模型。

DOI：

发表时间：

1989

期刊：

Blood

影响因子：

20.3

通讯作者：

中科院分区：

医学1区

文献类型：

作者：

L. Pegoraro;F. Malavasi;G. Bellone;M. Massaia;M. Boccadoro;G. Saglio;A. Guerrasio;G. Benetton;L. Lombardi;R. Coda

描述了从患有IgG-λ骨髓瘤的白血病转化患者的外周血衍生的人细胞系（LP-1）的特征。这些细胞类似于未成熟的浆细胞，因为它们表现出介于晚期B淋巴细胞和浆细胞之间的膜表型，尽管它们分泌IgG-λ链。用12-0十四烷酰佛波醇-13-乙酸酯（TPA）或商陆有丝分裂原（PWM）处理LP-1细胞诱导成熟浆细胞典型的表面标志物和超微结构特征的出现，但不影响其增殖活性。细胞系的分子分析显示c-myc原癌基因的表达增加和存在异常大小的转录本。传统的细胞遗传学和脉冲场凝胶电泳显示没有c-myc基因的结构重排，这表明异常c-myc表达可能是由于点突变或基因内的小缺失。LP-1细胞系是研究B细胞成熟过程的一个有用的模型;这种研究可能导致发现c-myc激活的不寻常机制。此外，LP-1细胞是产生人杂交瘤的潜在伴侣。

The characteristics of a human cell line (LP-1) derived from the peripheral blood of a patient with IgG-lambda myeloma in leukemic transformation are described. The cells resemble immature plasma cells in that they exhibit a membrane phenotype that is intermediate between late B lymphocytes and plasma cells, even though they secrete IgG-lambda chains. Treatment of LP-1 cells with 12-0 tetradecanoylphorbol-13-acetate (TPA) or pokeweek mitogen (PWM) induces the appearance of surface markers and ultrastructural features typical of mature plasma cells but does not affect their proliferative activity. Molecular analysis of the cell line showed an increased expression of the c-myc protooncogene and the presence of abnormally sized transcripts. Conventional cytogenetics and pulsed-field gel electrophoresis showed no structural rearrangements of the c-myc gene, suggesting that the abnormal c-myc expression may be due to point mutations or small deletions within the gene. The LP-1 cell line is a useful model in which to study the process of B-cell maturation; such study may lead to the uncovering of unusual mechanisms of c-myc activation. Furthermore, the LP-1 cell is a potential partner in the generation of human hybridomas.