Non-destructive mobile monitoring of microbial contaminations on meat surfaces using porphyrin fluorescence intensities.

Non-destructive mobile monitoring of microbial contaminations on meat surfaces using porphyrin fluorescence intensities.
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使用卟啉荧光强度对肉类表面微生物污染进行无损移动监测

DOI:
10.1016/j.meatsci.2015.12.022
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发表时间:
2016
期刊:
影响因子:
7.1
通讯作者:
Schlüter
Schlüter
中科院分区:
农林科学1区
文献类型:
--
作者:
Fröhling;Bolling;Thomasius;Schlüter

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本研究开发了一种移动的非破坏性肉品品质监测系统,并探讨了该系统在鲜肉生产全链条沿着的应用。将猪肉和羊肉在死后在5 °C下储存长达20天,并用荧光光谱仪测量。此外,通过不同的实验程序评估细菌对荧光信号的影响。NADH和不同卟啉的荧光可与不同细菌的生长相关,因此可用于污染监测。对于猪肉,卟啉荧光的增加在晚上9天后开始,对于羊肉,在下午2天后开始。在此基础上,建立了一套移动的荧光检测系统,并与实验室系统进行了比较。肉片的校正函数的均方根误差为1156.97 r.u.。平均绝对百分误差为12.59%,羔羊为470.81 r.u.。和15.55%。移动的和非侵入性的测量系统将提高鲜肉的微生物安全性。
A non-destructive mobile system for meat quality monitoring was developed and investigated for the possible application along the whole production chain of fresh meat. Pork and lamb meat was stored at 5 °C for up to 20 days post mortem and measured with a fluorescence spectrometer. Additionally, the bacterial influence on the fluorescence signals was evaluated by different experimental procedures. Fluorescence of NADH and different porphyrins could be correlated to the growth of diverse bacteria and hence used for contamination monitoring. The increase of porphyrin fluorescence started after 9 days p.m. for pork and after 2 days p.m. for lamb meat. Based on the results, a mobile fluorescence system was built and compared with the laboratory system. The corrected function of the meat slices showed a root mean square error of 1156.97 r.u. and a mean absolute percentage error of 12.59%; for lamb the values were 470.81 r.u. and 15.55%, respectively. A mobile and non-invasive measurement system would improve the microbial security of fresh meat.
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