Relationships between nitrogen cycling microbial community abundance and composition reveal the indirect effect of soil pH on oak decline.
Relationships between nitrogen cycling microbial community abundance and composition reveal the indirect effect of soil pH on oak decline.
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DOI：
10.1038/s41396-020-00801-0
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Whitby C
中科院分区：
文献类型：
--
作者：
Scarlett K;Denman S;Clark DR;Forster J;Vanguelova E;Brown N;Whitby C
树木减少是一个全球性的问题，其主要原因往往是未知的。氮（N）波动和酸化化合物之间的复杂相互作用已被认为是导致营养失衡和橡树抗逆性下降的因素。微生物在调节土壤氮素对植物的有效性方面起着至关重要的作用，但人们对土壤氮素循环与树木健康之间的关系知之甚少。在这里，我们将关键硝化和反硝化基因的高通量测序和qPCR分析与土壤化学分析相结合，以确定与症状（衰退）和无症状（表面健康）橡树（Quercus robur和Q. petraea）在英国。无症状的树木与较高丰度的AOB是由土壤pH值的积极驱动。AOA丰度和树的健康之间没有关系。然而，AOA丰度由较低浓度的NH4+驱动，进一步支持AOA有利于较低土壤NH4+浓度的想法。反硝化细菌丰度的影响主要是由土壤C：N比，并与AOB的相关性，无论树的健康。这些研究结果表明，通过平衡C：N改善土壤酸化可能会影响AOB丰度驱动N转化，减少对衰退橡树的压力。
Tree decline is a global concern and the primary cause is often unknown. Complex interactions between fluctuations in nitrogen (N) and acidifying compounds have been proposed as factors causing nutrient imbalances and decreasing stress tolerance of oak trees. Microorganisms are crucial in regulating soil N available to plants, yet little is known about the relationships between soil N-cycling and tree health. Here, we combined high-throughput sequencing and qPCR analysis of key nitrification and denitrification genes with soil chemical analyses to characterise ammonia-oxidising bacteria (AOB), archaea (AOA) and denitrifying communities in soils associated with symptomatic (declining) and asymptomatic (apparently healthy) oak trees (Quercus robur and Q. petraea) in the United Kingdom. Asymptomatic trees were associated with a higher abundance of AOB that is driven positively by soil pH. No relationship was found between AOA abundance and tree health. However, AOA abundance was driven by lower concentrations of NH4+, further supporting the idea of AOA favouring lower soil NH4+ concentrations. Denitrifier abundance was influenced primarily by soil C:N ratio, and correlations with AOB regardless of tree health. These findings indicate that amelioration of soil acidification by balancing C:N may affect AOB abundance driving N transformations, reducing stress on declining oak trees.
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