Developing a 3D intestinal epithelium model for livestock species.
Developing a 3D intestinal epithelium model for livestock species.
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DOI：
10.1007/s00441-018-2924-9
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发表时间：
2019-03
影响因子：
3.6
通讯作者：
Coombes JL
中科院分区：
文献类型：
--
作者：
Derricott H;Luu L;Fong WY;Hartley CS;Johnston LJ;Armstrong SD;Randle N;Duckworth CA;Campbell BJ;Wastling JM;Coombes JL
肠上皮的体外 3D 培养是研究其功能的宝贵资源。类器官培养利用干细胞再生和产生分化上皮的能力。来自啮齿动物或人体组织的肠道类器官模型已广泛使用，而大型动物模型则不然。牲畜肠道疾病和人畜共患疾病在动物和人类群体中引起显着的发病率和死亡率。因此，牲畜物种特异性模型可能为宿主-病原体相互作用和疾病反应提供新的见解。从屠宰场获得牛和猪的空肠，分离其肠隐窝，悬浮在基质胶中，培养，冷冻保存并复苏。隐窝“变圆”，随后类器官出芽，然后增大。使用免疫荧光染色和共聚焦显微镜对上皮细胞进行表征。类器官成功感染弓形虫或鼠伤寒沙门氏菌。这种 3D 类器官模型为研究各种病原体引起的物种特异性肠道感染提供了长期、可再生的资源。
The in vitro 3D culture of intestinal epithelium is a valuable resource in the study of its function. Organoid culture exploits stem cells’ ability to regenerate and produce differentiated epithelium. Intestinal organoid models from rodent or human tissue are widely available whereas large animal models are not. Livestock enteric and zoonotic diseases elicit significant morbidity and mortality in animal and human populations. Therefore, livestock species-specific models may offer novel insights into host-pathogen interactions and disease responses. Bovine and porcine jejunum were obtained from an abattoir and their intestinal crypts isolated, suspended in Matrigel, cultured, cryopreserved and resuscitated. ‘Rounding’ of crypts occurred followed by budding and then enlargement of the organoids. Epithelial cells were characterised using immunofluorescent staining and confocal microscopy. Organoids were successfully infected with Toxoplasma gondii or Salmonella typhimurium. This 3D organoid model offers a long-term, renewable resource for investigating species-specific intestinal infections with a variety of pathogens.
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影响因子：
4.5
作者：
Lemire S;Figueroa-Bossi N;Bossi L
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影响因子：
3.7
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DOI：
10.1073/pnas.1220272110
发表时间：
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影响因子：
11.1
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