Pellicle formation in Shewanella oneidensis.
Pellicle formation in Shewanella oneidensis.
复制标题
希瓦氏菌的菌膜形成
DOI：
10.1186/1471-2180-10-291
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发表时间：
2010-11-16
期刊：
影响因子：
4.2
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Liang Y;Gao H;Chen J;Dong Y;Wu L;He Z;Liu X;Qiu G;Zhou J
尽管近些年来，与固体表面相关的生物膜的发展已被广泛研究，但在气液界面形成的膜在很大程度上被忽视了。这项工作的目的是了解圆顶螺旋藻细胞膜形成的基本要求和机制。我们证明了当氧和某些阳离子存在时，膜的形成可以完成。钙(II)、锰(II)、铜(II)和锌(II)是从EDTA处理中完全挽救圆锥藻细胞膜形成所必需的元素，而镁(II)、铁(II)和铁(III)的作用较小。蛋白质而不是DNA是膜形成的关键，主要胞外多糖可能富含甘露糖。突变分析表明，膜的形成不需要鞭毛，但无鞭毛的突变体极大地延迟了膜的发育，这可能是由于在静态介质中生长减少所致。分析还表明，AggA-I型分泌系统是形成膜而不是与固体表面相关的生物膜所必需的。这种对膜形成的系统表征有助于我们对单氏葡萄球菌生物膜形成的理解，并表明膜可以作为研究细菌群落的良好模型。
Although solid surface-associated biofilm development of S. oneidensis has been extensively studied in recent years, pellicles formed at the air-liquid interface are largely overlooked. The goal of this work was to understand basic requirements and mechanism of pellicle formation in S. oneidensis. We demonstrated that pellicle formation can be completed when oxygen and certain cations were present. Ca(II), Mn(II), Cu(II), and Zn(II) were essential for the process evidenced by fully rescuing pellicle formation of S. oneidensis from the EDTA treatment while Mg (II), Fe(II), and Fe(III) were much less effective. Proteins rather than DNA were crucial in pellicle formation and the major exopolysaccharides may be rich in mannose. Mutational analysis revealed that flagella were not required for pellicle formation but flagellum-less mutants delayed pellicle development substantially, likely due to reduced growth in static media. The analysis also demonstrated that AggA type I secretion system was essential in formation of pellicles but not of solid surface-associated biofilms in S. oneidensis. This systematic characterization of pellicle formation shed lights on our understanding of biofilm formation in S. oneidensis and indicated that the pellicle may serve as a good research model for studying bacterial communities.
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影响因子：
3.4
作者：
De Vriendt, K;Theunissen, S;Van Beeumen, J
通讯作者：
Van Beeumen, J
影响因子：
5.1
作者：
McLean, J. S.;Pinchuk, G. E.;Beliaev, A. S.
通讯作者：
Beliaev, A. S.
影响因子：
4.4
作者：
Gao H;Wang X;Yang ZK;Palzkill T;Zhou J
通讯作者：
Zhou J
DOI：
10.1073/pnas.2334614100
发表时间：
2003-11-25
影响因子：
11.1
作者：
Kierek, K;Watnick, PI
通讯作者：
Watnick, PI
影响因子：
2.1
作者：
Moelling, Cas;Oberschlacke, Ross;Bergeron, Lori
通讯作者：
Bergeron, Lori