Basis of substrate binding and conservation of selectivity in the CLC family of channels and transporters.
Basis of substrate binding and conservation of selectivity in the CLC family of channels and transporters.
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DOI：
10.1038/nsmb.1704
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发表时间：
2009-12
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
--
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Ion binding to secondary active transporters triggers a cascade of conformational rearrangements resulting in substrate translocation across cellular membranes. Despite the fundamental role of this step, direct measurements of binding to transporters are rare. We investigated ion binding and selectivity in CLC-ec1, a H+/Cl− exchanger of the CLC family of channels and transporters. Cl− affinity depends on the conformation of the protein: it is highest with the extracellular gate removed, and weakens as the transporter adopts the occluded configuration and with the intracellular gate removed. The central ion-binding site determines selectivity in CLC transporters and channels, a serine to proline substitution at this site confers NO3− selectivity upon the Cl− specific CLC-ec1 transporter and CLC-0 channel. We propose that CLC-ec1 operates through an affinity-switch mechanism and that the bases of substrate specificity are conserved in the CLC channels and transporters.
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影响因子：
3.8
作者：
Accardi, Alessio;Walden, Michael;Nguitragool, Wang;Jayaram, Hariharan;Williams, Carole;Miller, Christopher
通讯作者：
Miller, Christopher
CLC Cl-/H+ 交换器中的细胞内质子转移突变体。
DOI：
10.1085/jgp.200810112
发表时间：
2009-02
期刊：
The Journal of general physiology
影响因子：
--
作者：
Lim HH;Miller C
通讯作者：
Miller C
影响因子：
64.8
作者：
Iyer, R;Iverson, TM;Miller, C
通讯作者：
Miller, C
影响因子：
64.8
作者：
Graves, Austin R.;Curran, Patricia K.;Mindell, Joseph A.
通讯作者：
Mindell, Joseph A.
影响因子：
56.9
作者：
Dutzler, R;Campbell, EB;MacKinnon, R
通讯作者：
MacKinnon, R