Advances in application of genome editing in tomato and recent development of genome editing technology.
Advances in application of genome editing in tomato and recent development of genome editing technology.
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DOI：
10.1007/s00122-021-03874-3
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Cheng Y
中科院分区：
文献类型：
--
作者：
Xia X;Cheng X;Li R;Yao J;Li Z;Cheng Y
基因组编辑是以CRISPR/Cas9系统为代表的分子生物学革命性技术，已广泛应用于植物基因功能表征和作物改良。番茄作为水果生物学研究的优秀模式植物，为人类饮食做出了巨大的营养贡献，是基因组编辑最重要的应用植物之一。使用 CRISPR/Cas9 介导的定向诱变，对果实成熟所必需的番茄基因的重新评估突出表明，应该重新考虑果实成熟的几个方面。基因组编辑也已应用于番茄育种，以提高果实产量和品质、增强抗逆性、加速野生番茄的驯化，以及最近为城市农业定制番茄品种。此外，基因组编辑不断创新，一些新的基因组编辑系统例如最近在精确基因组编辑方面取得突破的prime edit最近已在植物中得到应用。本文综述了基因组编辑在番茄中的应用进展以及基因组编辑技术的最新发展，并探讨了它们对植物研究和植物精准育种留下的重要启示。
Genome editing, a revolutionary technology in molecular biology and represented by the CRISPR/Cas9 system, has become widely used in plants for characterizing gene function and crop improvement. Tomato, serving as an excellent model plant for fruit biology research and making a substantial nutritional contribution to the human diet, is one of the most important applied plants for genome editing. Using CRISPR/Cas9-mediated targeted mutagenesis, the re-evaluation of tomato genes essential for fruit ripening highlights that several aspects of fruit ripening should be reconsidered. Genome editing has also been applied in tomato breeding for improving fruit yield and quality, increasing stress resistance, accelerating the domestication of wild tomato, and recently customizing tomato cultivars for urban agriculture. In addition, genome editing is continuously innovating, and several new genome editing systems such as the recent prime editing, a breakthrough in precise genome editing, have recently been applied in plants. In this review, these advances in application of genome editing in tomato and recent development of genome editing technology are summarized, and their leaving important enlightenment to plant research and precision plant breeding is also discussed.
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