IL-9 Inhibits Viral Replication in Coxsackievirus B3-Induced Myocarditis.
IL-9 Inhibits Viral Replication in Coxsackievirus B3-Induced Myocarditis.
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IL-9 抑制柯萨奇病毒 B3 诱发的心肌炎中的病毒复制
DOI：
10.3389/fimmu.2016.00409
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发表时间：
2016
影响因子：
7.3
通讯作者：
Cheng X
中科院分区：
文献类型：
--
作者：
Yu M;Long Q;Li HH;Liang W;Liao YH;Yuan J;Cheng X
病毒性心肌炎(VMC)的心肌损伤是由病毒感染和相关的自身免疫性疾病引起的。最近的研究表明，除了过敏性疾病外，IL-9还介导了抗微生物免疫和自身免疫反应。然而，IL-9在病毒感染和VMC中的作用仍然存在争议和不确定性。本研究用柯萨奇病毒B3(CVB3)感染Balb/c小鼠，发现病毒感染后第5天和第7天，VMC小鼠血液和心脏组织中IL-9含量较高。IL-9主要由CD8+T细胞在第5天和CD4+T细胞在第7天在心肌中分泌。此外，IL-9基因敲除加剧了CVB3感染后的心脏损害，伴随着病毒复制和IL-17a表达的急剧增加，以及转化生长因子-β的减少。相反，在感染CVB的Balb/c小鼠中，IL-9的重复表达导致了相反的效果。体外研究进一步表明，IL-9通过减少柯萨奇病毒和腺病毒受体的表达，直接抑制心肌细胞中病毒的复制，这可能与上调心肌细胞转化生长因子-β的自分泌作用有关。而IL-9对心肌细胞的凋亡无直接作用。我们的数据表明，IL-9在VMC的早期阶段通过抑制CVB3的复制而在疾病进展中起到保护作用。
Myocardial injuries in viral myocarditis (VMC) are caused by viral infection and related autoimmune disorders. Recent studies suggest that IL-9 mediated both antimicrobial immune and autoimmune responses in addition to allergic diseases. However, the role of IL-9 in viral infection and VMC remains controversial and uncertain. In this study, we infected Balb/c mice with Coxsackievirus B3 (CVB3), and found that IL-9 was enriched in the blood and hearts of VMC mice on days 5 and 7 after virus infection. Most of IL-9 was secreted by CD8+ T cells on day 5 and CD4+ T cells on day 7 in the myocardium. Further, IL-9 knockout exacerbated cardiac damage following CVB3 infection, along with a sharp increase in viral replication and IL-17a expression, as well as a decrease in TGF-β. In contrast, the repletion of IL-9 in Balb/c mice with CVB infection induced the opposite effect. Studies in vitro further revealed that IL-9 directly inhibited viral replication in cardiomyocytes by reducing coxsackie and adenovirus receptor expression, which might be associated with upregulation of TGF-β autocrine effect in these cells. However, IL-9 had no direct effect on apoptosis in cardiomyocytes. Our data indicated that IL-9 played a protective role in disease progression by inhibiting CVB3 replication in the early stages of VMC.
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柯萨奇病毒B3诱导的小鼠病毒性心肌炎中Th17和Th9细胞的明显差异表达
影响因子：
4.8
作者：
Qing K;Weifeng W;Fan Y;Yuluan Y;Yu P;Yanlan H
通讯作者：
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影响因子：
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5
作者：
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DOI：
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发表时间：
2007-07-01
影响因子：
4.8
作者：
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通讯作者：
Croze, Ed