Analyses of mitochondrial amino acid sequence datasets support the proposal that specimens of Hypodontus macropi from three species of macropodid hosts represent distinct species.
Analyses of mitochondrial amino acid sequence datasets support the proposal that specimens of Hypodontus macropi from three species of macropodid hosts represent distinct species.
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DOI：
10.1186/1471-2148-13-259
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发表时间：
2013-11-21
影响因子：
3.4
通讯作者：
Gasser RB
中科院分区：
文献类型：
--
作者：
Jabbar A;Beveridge I;Mohandas N;Chilton NB;Littlewood DT;Jex AR;Gasser RB
Hypodontus macropi 是一系列袋鼠和小袋鼠（巨足有袋动物）常见的肠道线虫。根据之前的多位点酶电泳 (MEE) 和核糖体 DNA 序列数据集，H. macropi 被认为是物种复杂的。为了使用独立的分子数据测试这一提议，我们对来自三种不同宿主物种（MacropusRobustususbustus、Thylogalebilardierii和Macropus[Wallabia]bicolor）以及Macropicolaocydromi（一种相关线虫）的H.macropi个体的整个线粒体（mt）基因组进行了测序，并对源自这些基因组的氨基酸序列数据集进行了比较分析。通过新一代 (454) 技术对来自三个宿主物种的 H. macropi 进行的 mt 基因组测序，其大小从 13,634 bp 到 13,699 bp 不等。对这三个 mt 基因组预测的氨基酸序列进行两两比较，结果显示存在 5.8% 至 18% 的差异。使用贝叶斯推理 (BI) 对氨基酸序列数据集进行的系统发育分析表明，来自三种不同宿主物种的 H. macropi 形成了独特的、得到良好支持的进化枝。此外，mt 基因组的滑动窗口分析为未来不同巨足宿主和澳大利亚各地地理区域中 H. macropi 的群体遗传学研究定义了可变区域。目前对推断的 mt 蛋白序列数据集的分析清楚地支持了这样的假设：来自 M.Robustusus、M.bicolor 和 T.bilardierii 的 H.macropi 代表不同的物种。
Hypodontus macropi is a common intestinal nematode of a range of kangaroos and wallabies (macropodid marsupials). Based on previous multilocus enzyme electrophoresis (MEE) and nuclear ribosomal DNA sequence data sets, H. macropi has been proposed to be complex of species. To test this proposal using independent molecular data, we sequenced the whole mitochondrial (mt) genomes of individuals of H. macropi from three different species of hosts (Macropus robustus robustus, Thylogale billardierii and Macropus [Wallabia] bicolor) as well as that of Macropicola ocydromi (a related nematode), and undertook a comparative analysis of the amino acid sequence datasets derived from these genomes. The mt genomes sequenced by next-generation (454) technology from H. macropi from the three host species varied from 13,634 bp to 13,699 bp in size. Pairwise comparisons of the amino acid sequences predicted from these three mt genomes revealed differences of 5.8% to 18%. Phylogenetic analysis of the amino acid sequence data sets using Bayesian Inference (BI) showed that H. macropi from the three different host species formed distinct, well-supported clades. In addition, sliding window analysis of the mt genomes defined variable regions for future population genetic studies of H. macropi in different macropodid hosts and geographical regions around Australia. The present analyses of inferred mt protein sequence datasets clearly supported the hypothesis that H. macropi from M. robustus robustus, M. bicolor and T. billardierii represent distinct species.
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影响因子：
4
作者：
CHILTON, NB;BEVERIDGE, I;ANDREWS, RH
通讯作者：
ANDREWS, RH
影响因子：
4
作者：
BEVERIDGE, I;CHILTON, NB;ANDREWS, RH
通讯作者：
ANDREWS, RH
影响因子：
1.6
作者：
BEVERIDGE, I
通讯作者：
BEVERIDGE, I
影响因子：
2.6
作者：
Gasser, RB
通讯作者：
Gasser, RB
DOI：
10.1098/rspb.1985.0096
发表时间：
1985-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BISHOP, MJ;FRIDAY, AE
通讯作者：
FRIDAY, AE