RNA-Seq Profiling Shows Divergent Gene Expression Patterns in Arabidopsis Grown under Different Densities.
RNA-Seq Profiling Shows Divergent Gene Expression Patterns in Arabidopsis Grown under Different Densities.
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RNA-Seq 分析显示不同密度下生长的拟南芥中不同的基因表达模式
DOI：
10.3389/fpls.2017.02001
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Guo D;Song X;Yuan M;Wang Z;Ge W;Wang L;Wang J;Wang X
在高密度(HD)条件下生长的植物经历了对水、养分和光的竞争加剧，可能导致大小、生物量、形态和生产力的变化。然而，还没有研究集中在全基因组表达模式和生长密度之间的关系。在这里，我们进行了全基因组RNA测序，以检测在低密度和高密度下生长的拟南芥的基因表达模式。在检测到的20,660个基因中，有98个基因在HD生长条件下表达增强，107个基因在HD生长条件下表达下调。进一步的分析表明，密度的变化对新陈代谢和刺激相关基因的影响最大。此外，HD生长导致了避荫表型，表现为向上生长和莲座叶减少。此外，GRXS3、GRXS4、GRXS5、GRXS7和GRXS8基因在高密度下显著下调，表明高密度主要通过硝酸盐限制影响植物的生长。
Plants growing under high-density (HD) conditions experience increased competition for water, nutrients, and light, possibly leading to changes in size, biomass, morphology, and productivity. However, no research has focused on the relationship between whole-genome expression patterns and growth density. Here, we performed whole-genome RNA sequencing to examine the gene expression patterns in Arabidopsis grown under low and high densities. Of the 20,660 detected genes, the expression levels of 98 were enhanced and 107 were repressed under HD growth. Further analysis revealed that changes in density influenced metabolism- and stimulus-related genes the most. Furthermore, HD growth led to a shade avoidance phenotype, represented by upward growth and a reduction in rosette leaves. Moreover, a cluster of glutaredoxin genes, GRXS3, 4, 5, 7, and 8, were significantly down-regulated under high density, suggesting that high density affects plant growth mainly by nitrate limitation.
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影响因子：
3.7
作者：
Song Y;Rui Y;Bedane G;Li J
通讯作者：
Li J
影响因子：
4.6
作者：
Kuai J;Sun Y;Zuo Q;Huang H;Liao Q;Wu C;Lu J;Wu J;Zhou G
通讯作者：
Zhou G
影响因子：
56.9
作者：
Wigge, PA;Kim, MC;Weigel, D
通讯作者：
Weigel, D
影响因子：
4.1
作者：
El-Zaeddi, Hussein;Martinez-Tome, Juan;Carbonell-Barrachina, Angel A.
通讯作者：
Carbonell-Barrachina, Angel A.
影响因子：
11.6
作者：
Gonzalez-Grandio, Eduardo;Poza-Carrion, Cesar;Cubas, Pilar
通讯作者：
Cubas, Pilar