Oral microbial community assembly under the influence of periodontitis.
Oral microbial community assembly under the influence of periodontitis.
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牙周炎影响下的口腔微生物群落组装
DOI：
10.1371/journal.pone.0182259
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Ma ZS
中科院分区：
文献类型：
--
作者：
Chen H;Peng S;Dai L;Zou Q;Yi B;Yang X;Ma ZS
自20世纪90年代以来，关于牙周炎病因的几种生态学假说（如特异性菌斑、非特异性菌斑和关键病原体）被提出，其中大多数都集中在与牙周炎相关的生态失调概念上。然而，现有的假说都没有对生态失调如何以及为什么发生提出机制解释。Hubbell的生物多样性中性理论提供了一个强大的零模型来检验宏基因组测序数据中关于群落组装和多样性维持机制的假设，这有助于理解形成群落动态的力量，如生态失调。在这里，我们重新分析Abusleme等人对牙周炎患者和健康个体的口腔微生物群落进行比较研究的数据集。我们的研究表明，61个群落中有14个（23%）通过了中性测试，这一比例显著高于之前报道的人类微生物组中1%的中性率（Li & Ma 2016, Scientific Reports）。这表明，虽然生态位选择可能在口腔微生物组的组装和多样性维持中起主导作用，但中性动力学的作用也不可忽视。然而，通过Fisher精确概率检验和多重检验校正，在牙周炎治疗组和健康治疗组之间的中性通良率没有统计学上的显著差异，这表明社区组装机制对牙周炎等干扰是强大的。此外，我们的研究证实了先前的发现，牙周炎患者具有更高的生物多样性。这些发现表明，虽然牙周炎可能会显著改变通过多样性测量的群落组成（即群落组装的展示或“表型”），但它似乎不会导致群落组装的“基因型”（机制）的“突变”。我们认为，“表型”变化解释了观察到的牙周炎和社区生态失调之间的联系（不一定是因果关系），这当然值得进一步研究。
Several ecological hypotheses (e.g., specific plaque, non-specific plaque and keystone pathogen) regarding the etiology of periodontitis have been proposed since the 1990s, most of which have been centered on the concept of dysbiosis associated with periodontitis. Nevertheless, none of the existing hypotheses have presented mechanistic interpretations on how and why dysbiosis actually occurs. Hubbell’s neutral theory of biodiversity offers a powerful null model to test hypothesis regarding the mechanism of community assembly and diversity maintenance from the metagenomic sequencing data, which can help to understand the forces that shape the community dynamics such as dysbiosis. Here we reanalyze the dataset from Abusleme et al.’s comparative study of the oral microbial communities from periodontitis patients and healthy individuals. Our study demonstrates that 14 out of 61 communities (23%) passed the neutrality test, a percentage significantly higher than the previous reported neutrality rate of 1% in human microbiome (Li & Ma 2016, Scientific Reports). This suggests that, while the niche selection may play a predominant role in the assembly and diversity maintenance in oral microbiome, the effect of neutral dynamics may not be ignored. However, no statistically significant differences in the neutrality passing rates were detected between the periodontitis and healthy treatments with Fisher’s exact probability test and multiple testing corrections, suggesting that the mechanism of community assembly is robust against disturbances such as periodontitis. In addition, our study confirmed previous finding that periodontitis patients exhibited higher biodiversity. These findings suggest that while periodontitis may significantly change the community composition measured by diversity (i.e., the exhibition or ‘phenotype’ of community assembly), it does not seem to cause the ‘mutation’ of the ‘genotype” (mechanism) of community assembly. We argue that the ‘phenotypic’ changes explain the observed link (not necessarily causal) between periodontitis and community dysbiosis, which is certainly worthy of further investigation.
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Keystone-Pathogen假设。
DOI：
10.1038/nrmicro2873
发表时间：
2012-10
期刊：
Nature reviews. Microbiology
影响因子：
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作者：
通讯作者：
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影响因子：
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作者：
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影响因子：
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通讯作者：
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影响因子：
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Li, Yi;Feng, Xianghui;Meng, Huanxin
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