Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome.
Vitamin A Deficiency Alters the Phototransduction Machinery and Distinct Non-Vision-Specific Pathways in the Drosophila Eye Proteome.
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DOI：
10.3390/biom12081083
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发表时间：
2022-08-06
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
已经在脊椎动物和无脊椎动物模型生物中研究了合成视觉发色团和光敏色素所需的维生素 A。 To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact that Drosophila melanogaster predominantly requires vitamin A for vision, but not for development or survival.我们分析了维生素 A 缺乏对果蝇眼睛的形态、脂质组和蛋白质组的影响。我们发现，长期缺乏维生素 A 会损害光感应室并导致视觉色素急剧丧失，但也会降低大多数放大和转导视觉信号的光转导蛋白的摩尔丰度。 Unexpectedly, vitamin A deficiency also decreased the abundances of specific subunits of mitochondrial TCA cycle and respiratory chain components but increased the levels of cuticle- and lens-related proteins.相比之下，我们发现维生素 A 缺乏对眼部脂质组没有明显影响。 In summary, chronic vitamin A deficiency decreases the levels of most components of the visual signaling pathway, but also affects molecular pathways that are not vision-specific and whose mechanistic connection to vitamin A remains to be elucidated.
The requirement of vitamin A for the synthesis of the visual chromophore and the light-sensing pigments has been studied in vertebrate and invertebrate model organisms. To identify the molecular mechanisms that orchestrate the ocular response to vitamin A deprivation, we took advantage of the fact that Drosophila melanogaster predominantly requires vitamin A for vision, but not for development or survival. We analyzed the impacts of vitamin A deficiency on the morphology, the lipidome, and the proteome of the Drosophila eye. We found that chronic vitamin A deprivation damaged the light-sensing compartments and caused a dramatic loss of visual pigments, but also decreased the molar abundance of most phototransduction proteins that amplify and transduce the visual signal. Unexpectedly, vitamin A deficiency also decreased the abundances of specific subunits of mitochondrial TCA cycle and respiratory chain components but increased the levels of cuticle- and lens-related proteins. In contrast, we found no apparent effects of vitamin A deficiency on the ocular lipidome. In summary, chronic vitamin A deficiency decreases the levels of most components of the visual signaling pathway, but also affects molecular pathways that are not vision-specific and whose mechanistic connection to vitamin A remains to be elucidated.
果蝇复眼中 3-羟基视黄醛和视觉色素的生物发生对光和维生素 A 衍生物的依赖性。
影响因子：
3.8
作者：
Isono, K;Tanimura, T;Oda, Y;Tsukahara, Y
通讯作者：
Tsukahara, Y