Behavioural rhythm splitting in the CS mouse is related to clock gene expression outside the suprachiasmatic nucleus
Behavioural rhythm splitting in the CS mouse is related to clock gene expression outside the suprachiasmatic nucleus
复制标题
CS小鼠的行为节律分裂与视交叉上核外的时钟基因表达有关
DOI：
--
复制
发表时间：
2001
影响因子：
3.4
通讯作者：
K. Honma
中科院分区：
文献类型：
--
作者：
H. Abe;S. Honma;M. Namihira;S. Masubuchi;K. Honma
CS小鼠表现出自发分裂的昼夜节律的运动活动在恒定的黑暗下，这表明它们包含两个弱耦合的振荡器在调节运动活动节律的生物钟系统。为了阐明这两个振荡器是否位于哺乳动物主昼夜节律起搏点所在的视交叉上核（SCN）中，我们通过原位杂交检测了小鼠SCN和大脑皮质中周期基因（mPer 1、mPer 2和mPer 3）mRNA的昼夜节律。在SCN中，mPer1和mPer2在分裂和未分裂小鼠中均显示出具有单峰的昼夜节律。mPer1和mPer2的节律在节律分裂过程中相对于活动开始略有相位延迟，但两种节律之间的相位关系没有改变。在大脑皮层，mPer1和mPer2的表达经历了双峰波动，峰值时间对应于分裂活动成分。未分裂小鼠的昼夜节律呈单峰型。分裂和未分裂小鼠之间的SCN或大脑皮层中的mPer3节律没有差异。这些结果表明，在SCN中的mPer1，mPer2和mPer3的昼夜振荡与CS小鼠的节律分裂无关。CS小鼠的分裂节律被认为是由位于SCN外部的振荡器与SCN昼夜节律起搏器解偶联引起的。
CS mice exhibit a spontaneous splitting in the circadian rhythm of locomotor activity under constant darkness, suggesting that they contain two weakly coupled oscillators in the circadian clock system regulating locomotor activity rhythm. In order to clarify whether the two oscillators are located in the suprachiasmatic nucleus (SCN), a site of the master circadian pacemaker in mammals, circadian rhythms in mRNA of mouse Period genes (mPer1, mPer2 and mPer3) in the SCN and cerebral cortex were examined during rhythm splitting by in situ hybridization. In the SCN, mPer1 and mPer2 showed a circadian rhythm with a single peak in both split and unsplit mice. The rhythms of mPer1 and mPer2 were slightly phase delayed during rhythm splitting in reference to the activity onset, but the phase relationship between the two rhythms was not changed. In the cerebral cortex, the expression of mPer1 and mPer2 underwent the bimodal fluctuation with peaks temporally corresponding to split activity components. The unsplit mice showed the circadian rhythms with a single peak. There was no difference in the mPer3 rhythms in either the SCN or the cerebral cortex between the split and unsplit mice. These results indicate that the circadian oscillations of mPer1, mPer2 and mPer3 in the SCN are not related to the rhythm splitting of CS mice. The split rhythms of the CS mice are suggested to be caused by uncoupling of oscillators located outside the SCN from the SCN circadian pacemaker.
DOI：
10.1073/pnas.96.21.12114
发表时间：
1999-10-12
影响因子：
11.1
作者：
Vitaterna, MH;Selby, CP;Sancar, A
通讯作者：
Sancar, A
影响因子：
56.9
作者：
Yamazaki, S;Numano, R;Tei, H
通讯作者：
Tei, H
影响因子：
56.9
作者：
de la Iglesia, HO;Meyer, J;Schwartz, WJ
通讯作者：
Schwartz, WJ