Counting actin in contractile rings reveals novel contributions of cofilin and type II myosins to fission yeast cytokinesis.
Counting actin in contractile rings reveals novel contributions of cofilin and type II myosins to fission yeast cytokinesis.
复制标题
计算收缩环中的肌动蛋白揭示了cofilin和II型肌球蛋白对分裂酵母细胞分裂的新贡献。
DOI：
10.1091/mbc.e21-08-0376
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发表时间：
2022-05-15
影响因子：
3.3
通讯作者：
Chen, Qian
中科院分区：
文献类型：
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作者：
Malla, Mamata;Pollard, Thomas D.;Chen, Qian
动物、真菌和变形虫的胞质分裂依赖于肌动球蛋白收缩环，肌动球蛋白收缩环由肌动蛋白丝的连续更新来稳定。关于收缩环中聚合肌动蛋白的量知之甚少，因此我们使用低浓度的GFP-Lifeact来计数活的裂变酵母细胞收缩环中的总聚合肌动蛋白分子。野生型细胞的收缩环以4900/min的速度积累聚合的肌动蛋白分子，达到198，000的峰值，随后在整个收缩环中以5400/min的速度损失肌动蛋白。在adf 1-M3突变细胞与cofilin，切断肌动蛋白丝差，收缩环积累聚合肌动蛋白的两倍，并最终有几乎两倍多的肌动蛋白沿着与II型肌球蛋白Myo 2，Myp 2，和Cdc 12的比例增加。虽然30%的adf 1-M3突变细胞未能完全收缩其环，其余的在野生型速率从环失去肌动蛋白。II型肌球蛋白Myo 2和Myp 2的突变减少了收缩环肌动蛋白丝的一半，并减缓了肌动蛋白从环中丢失的速度。
Cytokinesis by animals, fungi, and amoebas depends on actomyosin contractile rings, which are stabilized by continuous turnover of actin filaments. Remarkably little is known about the amount of polymerized actin in contractile rings, so we used low concentrations of GFP-Lifeact to count total polymerized actin molecules in the contractile rings of live fission yeast cells. Contractile rings of wild-type cells accumulated polymerized actin molecules at 4900/min to a peak number of ∼198,000 followed by a loss of actin at 5400/min throughout ring constriction. In adf1-M3 mutant cells with cofilin that severs actin filaments poorly, contractile rings accumulated polymerized actin at twice the normal rate and eventually had almost twofold more actin along with a proportional increase in type II myosins Myo2, Myp2, and formin Cdc12. Although 30% of adf1-M3 mutant cells failed to constrict their rings fully, the rest lost actin from the rings at the wild-type rates. Mutations of type II myosins Myo2 and Myp2 reduced contractile ring actin filaments by half and slowed the rate of actin loss from the rings.
DOI：
10.1083/jcb.146.2.453
发表时间：
1999-07-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Konzok A;Weber I;Simmeth E;Hacker U;Maniak M;Müller-Taubenberger A
通讯作者：
Müller-Taubenberger A