DAip1, a Dictyostelium homologue of the yeast actin-interacting protein 1, is involved in endocytosis, cytokinesis, and motility.
DAip1, a Dictyostelium homologue of the yeast actin-interacting protein 1, is involved in endocytosis, cytokinesis, and motility.
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DOI：
10.1083/jcb.146.2.453
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发表时间：
1999-07-26
期刊：
影响因子：
--
通讯作者：
Müller-Taubenberger A
中科院分区：
文献类型：
--
作者：
Konzok A;Weber I;Simmeth E;Hacker U;Maniak M;Müller-Taubenberger A
网柄苔藓-KD蛋白DAip1含有9个WD40重复序列，与酿酒酵母的肌动蛋白相互作用蛋白1 Aip1p同源，与线虫、绒泡菌和高等真核生物的相关蛋白同源。我们发现DAip1定位于细胞皮质中富含丝状肌动蛋白的动态区域：吞噬杯、巨噬小体、板脂和其他伪足。在表达绿色荧光蛋白(GFP)标记的DAip1的细胞中，该蛋白迅速重新分布到新形成的皮质突起中。通过基因替换产生的零突变和过表达DAip1来评估DAip1在体内的功能。DAip1缺失的细胞生长受损，其液体相摄取、吞噬和运动的速率与野生型相比降低。在DAip1缺失的细胞中，胞质分裂延长，并趋向于多核。根据DAip1过表达的类似结果和Latrunculin-A处理的效果，我们提出了DAip1在体内控制肌动蛋白解聚的功能，可能是通过与Cofilin的相互作用实现的。我们的数据表明，DAip1在皮质肌动蛋白网络的快速重塑过程中起着重要的调节作用。
The 64-kD protein DAip1 from Dictyostelium contains nine WD40-repeats and is homologous to the actin-interacting protein 1, Aip1p, from Saccharomyces cerevisiae, and to related proteins from Caenorhabditis, Physarum, and higher eukaryotes. We show that DAip1 is localized to dynamic regions of the cell cortex that are enriched in filamentous actin: phagocytic cups, macropinosomes, lamellipodia, and other pseudopodia. In cells expressing green fluorescent protein (GFP)-tagged DAip1, the protein rapidly redistributes into newly formed cortical protrusions. Functions of DAip1 in vivo were assessed using null mutants generated by gene replacement, and by overexpressing DAip1. DAip1-null cells are impaired in growth and their rates of fluid-phase uptake, phagocytosis, and movement are reduced in comparison to wild-type rates. Cytokinesis is prolonged in DAip1-null cells and they tend to become multinucleate. On the basis of similar results obtained by DAip1 overexpression and effects of latrunculin-A treatment, we propose a function for DAip1 in the control of actin depolymerization in vivo, probably through interaction with cofilin. Our data suggest that DAip1 plays an important regulatory role in the rapid remodeling of the cortical actin meshwork.
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DOI：
10.1038/nsb0195-28
发表时间：
1995-01-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
AMBERG, DC;BASART, E;BOTSTEIN, D
通讯作者：
BOTSTEIN, D
影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
影响因子：
4.8
作者：
AIZAWA, H;SUTOH, K;YAHARA, I
通讯作者：
YAHARA, I
肌动蛋白去聚合因子（ADF/Cofilin）提高了细丝流动率：基于肌动蛋白的运动的影响。
DOI：
10.1083/jcb.136.6.1307
发表时间：
1997-03-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carlier MF;Laurent V;Santolini J;Melki R;Didry D;Xia GX;Hong Y;Chua NH;Pantaloni D
通讯作者：
Pantaloni D
影响因子：
4.4
作者：
Adler, HJ;Winnicki, RS;Lomax, MI
通讯作者：
Lomax, MI