Pretreatment Strategies to Enhance Enzymatic Hydrolysis and Cellulosic Ethanol Production for Biorefinery of Corn Stover.
Pretreatment Strategies to Enhance Enzymatic Hydrolysis and Cellulosic Ethanol Production for Biorefinery of Corn Stover.
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增强玉米秸秆生物精炼酶解和纤维素乙醇生产的预处理策略
DOI：
10.3390/ijms232113163
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发表时间：
2022-10-29
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
利用玉米秸秆等木质纤维素生产生物乙醇以减少对化石燃料的需求已引起人们越来越大的兴趣，但大多数研究主要集中在如何提高乙醇产量，而对玉米秸秆的生物炼制关注较少。为实现玉米秸秆不同组分的综合利用，采用不同的预处理策略对玉米秸秆进行破碎，同时提高酶消化率和纤维素乙醇产量。结果表明，稀酸（DA）和碱性亚硫酸钠（ASS）相结合的预处理工艺能有效地将玉米秸秆中的三种主要成分，纤维素、半纤维素和木质素的混合物，木糖回收率达到93.0%，木质素去除率为85.0%。采用DA和ASS联合预处理后，在底物终浓度为18%（w/v）时，采用流加半同步糖化发酵（S-SSF）工艺，酶解72 h时纤维素转化率达到85.4%，乙醇浓度达到48.5 g/L。亚硫酸铵预处理可使木质素去除83.8%，酶解72 h后纤维素转化率达到86.6%，乙醇浓度达到50 g/L。研究结果为有效分离玉米秸秆中的半纤维素和木质素以及利用玉米秸秆生物炼制纤维素乙醇提供了参考。
There is a rising interest in bioethanol production from lignocellulose such as corn stover to decrease the need for fossil fuels, but most research mainly focuses on how to improve ethanol yield and pays less attention to the biorefinery of corn stover. To realize the utilization of different components of corn stover in this study, different pretreatment strategies were used to fractionate corn stover while enhancing enzymatic digestibility and cellulosic ethanol production. It was found that the pretreatment process combining dilute acid (DA) and alkaline sodium sulfite (ASS) could effectively fractionate the three main components of corn stover, i.e., cellulose, hemicellulose, and lignin, that xylose recovery reached 93.0%, and that removal rate of lignin was 85.0%. After the joint pretreatment of DA and ASS, the conversion of cellulose at 72 h of enzymatic hydrolysis reached 85.4%, and ethanol concentration reached 48.5 g/L through fed-batch semi-simultaneous saccharification and fermentation (S-SSF) process when the final concentration of substrate was 18% (w/v). Pretreatment with ammonium sulfite resulted in 83.8% of lignin removal, and the conversion of cellulose and ethanol concentration reached 86.6% and 50 g/L after enzymatic hydrolysis of 72 h and fed-batch S-SSF, respectively. The results provided a reference for effectively separating hemicellulose and lignin from corn stover and producing cellulosic ethanol for the biorefinery of corn stover.
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