The response regulator SypE controls biofilm formation and colonization through phosphorylation of the syp-encoded regulator SypA in Vibrio fischeri.
The response regulator SypE controls biofilm formation and colonization through phosphorylation of the syp-encoded regulator SypA in Vibrio fischeri.
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DOI：
10.1111/mmi.12109
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发表时间：
2013-02
影响因子：
3.6
通讯作者：
Visick KL
中科院分区：
文献类型：
--
作者：
Morris AR;Visick KL
细菌利用多种调控系统来调节基因表达以应对环境变化，包括双组分信号系统和伴侣转换网络。我们最近发现了一种新的调节蛋白SypE，它结合了这两种信号系统的特征。SypE包含一个中央反应调节受体结构域，两侧是与伴侣转换蛋白相似的推定激酶和磷酸酶效应结构域。SypE先前被证明通过其末端效应域的相反活动对生物膜的形成施加双重控制。在这里，我们证明了SypE通过调节SypA的活性来控制费氏弧菌的生物膜，SypA是一种STAS（硫酸盐转运体和抗sigma拮抗剂）结构域蛋白。通过生物化学和遗传学方法，我们确定SypE可以使SypA磷酸化和去磷酸化，并且磷酸化抑制SypA的活性。此外，我们发现生物膜的形成和共生定植需要活性的、未磷酸化的SypA，因此SypA磷酸化与生物膜的丧失和宿主定植受损相对应。最后，SypA非磷酸化突变体的表达抑制了组成型抑制SypE突变株的生物膜和共生缺陷。本研究表明，SypE对SypA活性的调控是费氏弧菌控制生物膜发育和共生定植的重要机制。
Bacteria utilize multiple regulatory systems to modulate gene expression in response to environmental changes, including two-component signaling systems and partner-switching networks. We recently identified a novel regulatory protein SypE that combines features of both signaling systems. SypE contains a central response regulator receiver domain flanked by putative kinase and phosphatase effector domains with similarity to partner-switching proteins. SypE was previously shown to exert dual control over biofilm formation through the opposing activities of its terminal effector domains. Here, we demonstrate that SypE controls biofilms in Vibrio fischeri by regulating the activity of SypA, a STAS (sulphate transporter and anti-sigma antagonist) domain protein. Using biochemical and genetic approaches, we determined that SypE both phosphorylates and dephosphorylates SypA, and that phosphorylation inhibits SypA’s activity. Furthermore, we found that biofilm formation and symbiotic colonization required active, unphosphorylated SypA, and thus SypA phosphorylation corresponded with a loss of biofilms and impaired host colonization. Finally, expression of a non-phosphorylatable mutant of SypA suppressed both the biofilm and symbiosis defects of a constitutively inhibitory SypE mutant strain. This study demonstrates that regulation of SypA activity by SypE is a critical mechanism by which V. fischeri controls biofilm development and symbiotic colonization.
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影响因子：
10.5
作者：
DIEDERICH, B;WILKINSON, JF;YUDKIN, MD
通讯作者：
YUDKIN, MD
影响因子：
3.6
作者：
Mattoo, S;Yuk, MH;Miller, JF
通讯作者：
Miller, JF
影响因子：
3.2
作者：
Shibata, Satoshi;Visick, Karen L.
通讯作者：
Visick, Karen L.
DOI：
10.1073/pnas.97.18.10231
发表时间：
2000-08-29
影响因子：
11.1
作者：
Nyholm, SV;Stabb, EV;McFall-Ngai, MJ
通讯作者：
McFall-Ngai, MJ
影响因子：
4.4
作者：
McCann, J;Stabb, EV;Ruby, EG
通讯作者：
Ruby, EG