DNA dendrimer: an efficient nanocarrier of functional nucleic acids for intracellular molecular sensing.
DNA dendrimer: an efficient nanocarrier of functional nucleic acids for intracellular molecular sensing.
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DNA Dendrimer：一种用于细胞内分子传感的功能性核酸的高效纳米载体
DOI：
10.1021/nn5015962
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发表时间：
2014-06-24
期刊：
影响因子：
17.1
通讯作者：
Tan, Weihong
中科院分区：
文献类型：
--
作者：
Meng, Hong-Min;Zhang, Xiaobing;Lv, Yifan;Zhao, Zilong;Wang, Nan-Nan;Fu, Ting;Fan, Huanhuan;Liang, Hao;Qiu, Liping;Zhu, Guizhi;Tan, Weihong
功能性核酸（FNA）为基础的传感系统已经开发了一个有效的检测范围广泛的生物相关的分析物，采用DNA酶或适配体作为识别单元。然而，它们的细胞内递送一直是一个问题，主要是递送效率、动力学和递送的FNA的量。在这里，我们报告了一种DNA树枝状聚合物支架作为一种有效的纳米载体，提供FNA和进行原位监测的生物分子在活细胞中。分别选择组氨酸依赖性DNAzyme和抗ATP适体作为模型FNA以制备FNA树枝状聚合物。在细胞环境中，嵌入DNA树状聚合物的DNA酶的催化活性或对ATP的适体识别功能保持不变，灵敏度或特异性没有变化。此外，这些DNA树枝状纳米载体显示出优异的生物相容性、高细胞内递送效率和在细胞环境中的足够稳定性。这种FNA树枝状纳米载体可以在生物医学诊断和治疗中找到广泛的应用。
Functional nucleic acid (FNA)-based sensing systems have been developed for efficient detection of a wide range of biorelated analytes by employing DNAzymes or aptamers as recognition units. However, their intracellular delivery has always been a concern, mainly in delivery efficiency, kinetics, and the amount of delivered FNAs. Here we report a DNA dendrimer scaffold as an efficient nanocarrier to deliver FNAs and to conduct in situ monitoring of biological molecules in living cells. A histidine-dependent DNAzyme and an anti-ATP aptamer were chosen separately as the model FNAs to make the FNA dendrimer. The FNA-embedded DNA dendrimers maintained the catalytic activity of the DNAzyme or the aptamer recognition function toward ATP in the cellular environment, with no change in sensitivity or specificity. Moreover, these DNA dendrimeric nanocarriers show excellent biocompatibility, high intracellular delivery efficiency, and sufficient stability in a cellular environment. This FNA dendrimeric nanocarrier may find a broad spectrum of applications in biomedical diagnosis and therapy.
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影响因子：
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